Antioxidant response of vitamin A during the exposure of blood platelets to electromagnetic radiation generated by LCD monitors - in vitro study

Abstract

The article presents the results of in vitro studies aimed at identifying changes in activity of the enzyme superoxide dismutase (SOD-1) as a parameter of oxidative stress and protective antioxidant role of vitamin A during the exposure of blood platelets to electromagnetic radiation (EMR) generated by LCD monitors. Blood platelets were exposed to an electromagnetic radiation for 30 min. and 60 min. generated by monitors, which is characterized by parameters: 1 kHz frequency and 220 V/m intensity. The enzymatic activity of SOD-1 increases significantly compared to control values after 30 min. of exposure to EMR (from 2523.39 U/g protein to 3896.15 U/g protein), and decreases after 60 min (to 2846.58 U/g protein). A significant decrease in enzyme activity after the addition of vitamin A was noticed (to 1569.54 U/g protein). In samples exposed for 30 min. the SOD activity was significantly increased by addition of vitamin A and decreases after 60 min. Changes in enzymatic activity of SOD-1 dependent on exposure time and application of vitamin A suggest an important preventive role of vitamin A to protect against the effects of EMR which we are exposed to in everyday life.