Pub Date : 2022-04-27DOI: 10.1101/2022.04.25.22274272
A. Atkulwar, A. Rehman, Y. Imaan, M. Baig
This is the first study on omicron genomes from India to focus on phylodynamics and phylogenomics trait to provide an insight into the evolution of omicron variants. We analyzed 564 genomes deposited to GISAID database from various states of India. Pangolin COVID-19 Lineage Assigner tool was used to determine lineage assignment of all retrieved genomes. A Maximum likelihood (MLE) tree construction further confirms the separation of genomes into two distinct clades, BA. 1. and BA. 2. A very high reproduction number (R0) of 2.445 was estimated for the lineage BA.2. The highest R0 value in Telangana confirms the prevalence of lineage BA.2 in the state. Construction of the Reduced Median (RM) network shows evolution of some autochthonous haplogroups and haplotypes, which further supports the rapid evolution of omicron as compared to its previous variants. Phylogenomic analyses using maximum likelihood (ML) and RM show the potential for the emergence of sub-sublineages and novel haplogroups respectively. Due to the recombinant property and high transmissibility of omicron virus, we suggest continuous and more widespread genome sequencing in all states of India to track evolution of SARS-CoV-2 in real time.
{"title":"Analyses of Omicron genomes from India reveal BA.2 as a more transmissible variant","authors":"A. Atkulwar, A. Rehman, Y. Imaan, M. Baig","doi":"10.1101/2022.04.25.22274272","DOIUrl":"https://doi.org/10.1101/2022.04.25.22274272","url":null,"abstract":"This is the first study on omicron genomes from India to focus on phylodynamics and phylogenomics trait to provide an insight into the evolution of omicron variants. We analyzed 564 genomes deposited to GISAID database from various states of India. Pangolin COVID-19 Lineage Assigner tool was used to determine lineage assignment of all retrieved genomes. A Maximum likelihood (MLE) tree construction further confirms the separation of genomes into two distinct clades, BA. 1. and BA. 2. A very high reproduction number (R0) of 2.445 was estimated for the lineage BA.2. The highest R0 value in Telangana confirms the prevalence of lineage BA.2 in the state. Construction of the Reduced Median (RM) network shows evolution of some autochthonous haplogroups and haplotypes, which further supports the rapid evolution of omicron as compared to its previous variants. Phylogenomic analyses using maximum likelihood (ML) and RM show the potential for the emergence of sub-sublineages and novel haplogroups respectively. Due to the recombinant property and high transmissibility of omicron virus, we suggest continuous and more widespread genome sequencing in all states of India to track evolution of SARS-CoV-2 in real time.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"75 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86068796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shkar Rzgar K. Rostam, K. Shekhany, Harem O. Smail
Nowadays, inhaled allergens are the main causes of allergic diseases, which are derived from different sources such as animal dander, grasses, tree, insects and fungi/molds. Identification and detection of allergens play a critical role in the diagnosis and treatment of many allergic diseases. Aims were to determine the prevalence of most common inhaled allergens in Erbil province and determination the intensity of allergic response among allergic patients against 35 identified inhaled allergens items. A total number of 170 patients suffering from suspected inhalant allergy were checked in the present study. The study was carried out for patients who visited the private clinical sectors between 2018-2020 in Erbil province, Kurdistan Region of Iraq. Determination of specific IgE (sIgE) antibodies was examined for suspected patients. The country-specific inhaled allergy profile “Euroline inhaled Iraq 1” (Catalog no: DP 313816011 E, IVD approved, and CE certified EUROLINE immunoblot), containing strip for 35 different inhalant allergens, has been used in this study. Positive specific IgE to inhaled allergens was detected in 22.35% of our suspected patients. Orchard grass (21.05%) was the most inhaled allergen in our 38 allergic patients, followed by the Meadow foxtail (15.78%), Cockroach German and Sweet vernal grass (13.15%). Based on the present study results, we conclude that the prevalence of inhaled allergy differed between men and women in different age groups. Our study reached that there were no associations between inhaled allergens and sex or age. DOI: http://dx.doi.org/10.5281/zenodo.4395149
如今，吸入性过敏原是过敏性疾病的主要原因，其来源不同，如动物皮屑、草、树、昆虫和真菌/霉菌。过敏原的识别和检测在许多过敏性疾病的诊断和治疗中起着至关重要的作用。目的是确定埃尔比勒省最常见的吸入过敏原的患病率，并确定过敏患者对35种已确定的吸入过敏原的过敏反应强度。本研究共检查了170例疑似吸入性过敏患者。该研究是对2018-2020年在伊拉克库尔德斯坦地区埃尔比勒省访问私营临床部门的患者进行的。对疑似患者检测特异性IgE (sIgE)抗体。本研究使用了国家特异性吸入性过敏谱“Euroline吸入性伊拉克1号”(目录号:DP 313816011 E, IVD批准，CE认证的Euroline免疫印迹)，包含35种不同的吸入性过敏原的条带。对吸入过敏原特异性IgE阳性的疑似患者占22.35%。38例过敏患者吸入最多的过敏原是果园草(21.05%)，其次是草地狐尾草(15.78%)、德国蜚蠊和甜春草(13.15%)。根据目前的研究结果，我们得出结论，吸入性过敏的患病率在不同年龄组的男性和女性之间存在差异。我们的研究表明，吸入的过敏原与性别或年龄没有关联。DOI: http://dx.doi.org/10.5281/zenodo.4395149
{"title":"Prevalence of common inhaled allergies in Erbil province, Kurdistan Region of Iraq","authors":"Shkar Rzgar K. Rostam, K. Shekhany, Harem O. Smail","doi":"10.5281/ZENODO.4395149","DOIUrl":"https://doi.org/10.5281/ZENODO.4395149","url":null,"abstract":"Nowadays, inhaled allergens are the main causes of allergic diseases, which are derived from different sources such as animal dander, grasses, tree, insects and fungi/molds. Identification and detection of allergens play a critical role in the diagnosis and treatment of many allergic diseases. Aims were to determine the prevalence of most common inhaled allergens in Erbil province and determination the intensity of allergic response among allergic patients against 35 identified inhaled allergens items. A total number of 170 patients suffering from suspected inhalant allergy were checked in the present study. The study was carried out for patients who visited the private clinical sectors between 2018-2020 in Erbil province, Kurdistan Region of Iraq. Determination of specific IgE (sIgE) antibodies was examined for suspected patients. The country-specific inhaled allergy profile “Euroline inhaled Iraq 1” (Catalog no: DP 313816011 E, IVD approved, and CE certified EUROLINE immunoblot), containing strip for 35 different inhalant allergens, has been used in this study. Positive specific IgE to inhaled allergens was detected in 22.35% of our suspected patients. Orchard grass (21.05%) was the most inhaled allergen in our 38 allergic patients, followed by the Meadow foxtail (15.78%), Cockroach German and Sweet vernal grass (13.15%). Based on the present study results, we conclude that the prevalence of inhaled allergy differed between men and women in different age groups. Our study reached that there were no associations between inhaled allergens and sex or age. \u0000DOI: http://dx.doi.org/10.5281/zenodo.4395149","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"2 1","pages":"134-145"},"PeriodicalIF":0.0,"publicationDate":"2021-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81940100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Flood increases due to an increase in river overflow which affects on abiotic and biotic factors. The preliminary study of flood-affected crops was carried out in flood-affected cropland of Koshi Tappu Region of Eastern Nepal. For the experiment the plant residues of Eichhornia crassipes and Sesbania rostrata and inorganic fertilizer were selected to examine the growth and yield in Vigna unguiculata. The appropriate treatments for the production of V. unguiculata were analyzed. Before applying treatments, soil was collected and analyzed for physicochemical, microbial biomass and available nitrogen. Soil texture, soil moisture, water holding capacity and bulk density (BD) were calculated. The parameters such as soil pH organic carbon, organic matter and total nitrogen were determined. Soil microbe increases the significance of organic carbon and soil nitrogen is correlated for growth and yield. The results showed that the combined urea and plant residue increases the highest yield. And the Eichhornia compost represents the highest leaf area index and biomass. The total pod production was found in the Echhhornia compost. The dry weight per single pod in Eichhornia fresh was 7.82 g and in Sesbania fresh was 7.42 g. It proves that the land pattern is significant for the soil organic compounds. The experiment showed that the use of plant residues enhanced the increase of physicochemical properties of soil by adding the nutrients. The combined Urea + Eichhornia supports the best growth and development of the plant. DOI: http://dx.doi.org/10.5281/zenodo.4338159
{"title":"Establishment of plant residues and inorganic fertilizer application for growth and yield of Vigna unguiculata (L.) in flood-affected cropland of Koshi Tappu Region, Eastern Nepal","authors":"N. Paudel, S. Subedi, T. Mandal, Bishnu Dev Das","doi":"10.5281/ZENODO.4338159","DOIUrl":"https://doi.org/10.5281/ZENODO.4338159","url":null,"abstract":"Flood increases due to an increase in river overflow which affects on abiotic and biotic factors. The preliminary study of flood-affected crops was carried out in flood-affected cropland of Koshi Tappu Region of Eastern Nepal. For the experiment the plant residues of Eichhornia crassipes and Sesbania rostrata and inorganic fertilizer were selected to examine the growth and yield in Vigna unguiculata. The appropriate treatments for the production of V. unguiculata were analyzed. Before applying treatments, soil was collected and analyzed for physicochemical, microbial biomass and available nitrogen. Soil texture, soil moisture, water holding capacity and bulk density (BD) were calculated. The parameters such as soil pH organic carbon, organic matter and total nitrogen were determined. Soil microbe increases the significance of organic carbon and soil nitrogen is correlated for growth and yield. The results showed that the combined urea and plant residue increases the highest yield. And the Eichhornia compost represents the highest leaf area index and biomass. The total pod production was found in the Echhhornia compost. The dry weight per single pod in Eichhornia fresh was 7.82 g and in Sesbania fresh was 7.42 g. It proves that the land pattern is significant for the soil organic compounds. The experiment showed that the use of plant residues enhanced the increase of physicochemical properties of soil by adding the nutrients. The combined Urea + Eichhornia supports the best growth and development of the plant. \u0000DOI: http://dx.doi.org/10.5281/zenodo.4338159","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"8 1","pages":"75-87"},"PeriodicalIF":0.0,"publicationDate":"2021-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87190331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alzheimer’s disease (AD) is an irreversible and progressive neurodegenerative disease which affects about over 30 million people worldwide. There is no suitable treatment for AD nowadays. The current scenario of the research in the field of the search for suitable therapeutic approaches for the treatment of Alzheimer’s disease should be a shift towards the combinatorial approach of Ayurveda and Yoga. This review is mainly focused on to adapt Ayurveda and Yoga approaches for the treatment of Alzheimer’s disease. DOI: http://dx.doi.org/10.5281/zenodo.4320957
{"title":"Ayurveda and Yoga practices: a synergistic approach for the treatment of Alzheimer’s disease","authors":"S. Mishra, S. Singh","doi":"10.5281/ZENODO.4320957","DOIUrl":"https://doi.org/10.5281/ZENODO.4320957","url":null,"abstract":"Alzheimer’s disease (AD) is an irreversible and progressive neurodegenerative disease which affects about over 30 million people worldwide. There is no suitable treatment for AD nowadays. The current scenario of the research in the field of the search for suitable therapeutic approaches for the treatment of Alzheimer’s disease should be a shift towards the combinatorial approach of Ayurveda and Yoga. This review is mainly focused on to adapt Ayurveda and Yoga approaches for the treatment of Alzheimer’s disease. \u0000DOI: http://dx.doi.org/10.5281/zenodo.4320957","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"45 1","pages":"65-74"},"PeriodicalIF":0.0,"publicationDate":"2021-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85832271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asmaa Chbel, Aurelio Serrano Delgado, A. Soukri, B. E. Khalfi
The marine environment is characterized by a wide diversity of microorganisms among which marine bacteria. To insure their survival in hostile conditions where they face high competition with pathogenic microorganisms, they produce various kinds of bioactive molecules within biofilms with unique structural and functional features. As example: marine peptides which provide a broad spectrum of antimicrobial, antitumoral, antiviral and anti-inflammatory activities, in addition to marine exopolysaccharides showing antifouling and antifungal activities, immunomodulatory properties, emulsion stabilization capacity with other various potentials. Some biofilms have shown a beneficial role for aquaculture, among which enhancement of growth performance and improvement of water quality, while others are threatening not only aquaculture and maritime fields, but also medicine and food industry. Thus, marine bioactive compounds are promising preventing agents for the establishment and growth of fouling microorganisms, which may be useful in different fields in order to decrease economic losses and avoid foodborne illnesses. DOI: http://dx.doi.org/10.5281/zenodo.4384158
{"title":"Marine biomolecules: a promising approach in therapy and biotechnology","authors":"Asmaa Chbel, Aurelio Serrano Delgado, A. Soukri, B. E. Khalfi","doi":"10.5281/ZENODO.4384158","DOIUrl":"https://doi.org/10.5281/ZENODO.4384158","url":null,"abstract":"The marine environment is characterized by a wide diversity of microorganisms among which marine bacteria. To insure their survival in hostile conditions where they face high competition with pathogenic microorganisms, they produce various kinds of bioactive molecules within biofilms with unique structural and functional features. As example: marine peptides which provide a broad spectrum of antimicrobial, antitumoral, antiviral and anti-inflammatory activities, in addition to marine exopolysaccharides showing antifouling and antifungal activities, immunomodulatory properties, emulsion stabilization capacity with other various potentials. Some biofilms have shown a beneficial role for aquaculture, among which enhancement of growth performance and improvement of water quality, while others are threatening not only aquaculture and maritime fields, but also medicine and food industry. Thus, marine bioactive compounds are promising preventing agents for the establishment and growth of fouling microorganisms, which may be useful in different fields in order to decrease economic losses and avoid foodborne illnesses. DOI: http://dx.doi.org/10.5281/zenodo.4384158","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"32 1","pages":"122-133"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82610560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
SARS-CoV-2, the mighty manslayer, responsible for COVID-19, has currently killed over 1.54 million people worldwide and 141,000 in India alone. It has affected around 67 million people globally and 9.68 million in India. It has quarantined the whole world. Doctors and scientists are working around the clock to save the world from this deadly virus. Since the number of patients is increasing rapidly, it is essential to test as many suspects as possible. But with the diagnostic tests that are being used currently, the polymerase chain reaction, antibody detection (Serological tests), Rapid Diagnostic tests (RDT), antigen tests and Isothermal Amplification assays are time consuming and there is a high chance that the test might come back with the wrong results. SHERLOCK and DETECTR are CRISPR-based diagnostic tool that were recently worked upon and showed very promising results. The test results come back in less than 40 minutes and the tests are far more accurate than all of the current diagnostics which makes them far more efficient than the others.
{"title":"SHERLOCK and DETECTR CRISPR-CAS systems as better diagnostic tools for COVID-19","authors":"Salai S. Sumukhi, E. Joseph","doi":"10.5281/zenodo.5237359","DOIUrl":"https://doi.org/10.5281/zenodo.5237359","url":null,"abstract":"SARS-CoV-2, the mighty manslayer, responsible for COVID-19, has currently killed over 1.54 million people worldwide and 141,000 in India alone. It has affected around 67 million people globally and 9.68 million in India. It has quarantined the whole world. Doctors and scientists are working around the clock to save the world from this deadly virus. Since the number of patients is increasing rapidly, it is essential to test as many suspects as possible. But with the diagnostic tests that are being used currently, the polymerase chain reaction, antibody detection (Serological tests), Rapid Diagnostic tests (RDT), antigen tests and Isothermal Amplification assays are time consuming and there is a high chance that the test might come back with the wrong results. SHERLOCK and DETECTR are CRISPR-based diagnostic tool that were recently worked upon and showed very promising results. The test results come back in less than 40 minutes and the tests are far more accurate than all of the current diagnostics which makes them far more efficient than the others.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"1 1","pages":"392-403"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89671676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The ongoing pandemic has been increasing slowly and steadily across the world. The SARS-CoV-2 spreads through droplet disseminated from infected persons via coughing and/or sneezing onto the face, nasopharyngeal, and oropharyngeal mucosa. In order to prevent the transmission of coronavirus disease, WHO and public health officials made policies, advised the health workers and public to wear face coverings (masks). The nature of masks depends upon the source, material, structure and particulate efficacies. The main objective of this study is to provide information about efficacies of different types of masks used during COVID-19 pandemic.
{"title":"Types of face coverings (masks) and Coronavirus disease 2019 (COVID-19)","authors":"Anjum Tajamul Islam Summer","doi":"10.5281/zenodo.5068415","DOIUrl":"https://doi.org/10.5281/zenodo.5068415","url":null,"abstract":"The ongoing pandemic has been increasing slowly and steadily across the world. The SARS-CoV-2 spreads through droplet disseminated from infected persons via coughing and/or sneezing onto the face, nasopharyngeal, and oropharyngeal mucosa. In order to prevent the transmission of coronavirus disease, WHO and public health officials made policies, advised the health workers and public to wear face coverings (masks). The nature of masks depends upon the source, material, structure and particulate efficacies. The main objective of this study is to provide information about efficacies of different types of masks used during COVID-19 pandemic.","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"35 1","pages":"325-331"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75747104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Rahman, S. Hossain, N. Abdullah, Norhaniza Aminudin
Ganoderma lucidum has been hailed as medicinal mushroom. Its effect on memory and learning related behavioral performance along with related protein markers has been evaluated using Alzheimer’s disease (AD) and hypercholesterolemic model rats in the present study. AD model rats were prepared infusing amyloid beta peptide into the right ventricles of the rats. Hypercholesterolemia was evoked feeding 1% cholesterol and 1% cholic acid with basal diet of the rats for 8 weeks. Hot water extract of G. lucidum was ingested orally (200 mg/kg bw) to the HC and AD model rats. Memory and learning related behavioral tests were performed using Barnes maze while protein markers (BDNF, SNAP2, PSD-95, VAchT) were detected using ELISA. Observed findings suggest hypocholesterolemic, lipid profile improving and enhanced cognitive performance of the G. lucidum fed rats. Memory and learning related protein markers also substantiate this fruition. Thus, therapeutic potentiality of Ganoderma lucidum in AD amelioration seems promising. DOI: http://dx.doi.org/10.5281/zenodo.4009588
{"title":"Validation of Ganoderma lucidum against hypercholesterolemia and Alzheimer's disease","authors":"M. Rahman, S. Hossain, N. Abdullah, Norhaniza Aminudin","doi":"10.5281/ZENODO.4009588","DOIUrl":"https://doi.org/10.5281/ZENODO.4009588","url":null,"abstract":"Ganoderma lucidum has been hailed as medicinal mushroom. Its effect on memory and learning related behavioral performance along with related protein markers has been evaluated using Alzheimer’s disease (AD) and hypercholesterolemic model rats in the present study. AD model rats were prepared infusing amyloid beta peptide into the right ventricles of the rats. Hypercholesterolemia was evoked feeding 1% cholesterol and 1% cholic acid with basal diet of the rats for 8 weeks. Hot water extract of G. lucidum was ingested orally (200 mg/kg bw) to the HC and AD model rats. Memory and learning related behavioral tests were performed using Barnes maze while protein markers (BDNF, SNAP2, PSD-95, VAchT) were detected using ELISA. Observed findings suggest hypocholesterolemic, lipid profile improving and enhanced cognitive performance of the G. lucidum fed rats. Memory and learning related protein markers also substantiate this fruition. Thus, therapeutic potentiality of Ganoderma lucidum in AD amelioration seems promising. \u0000DOI: http://dx.doi.org/10.5281/zenodo.4009588","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"24 1","pages":"314-325"},"PeriodicalIF":0.0,"publicationDate":"2020-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77512826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Srivastav, S. Srivastava, Abhishek Kumar, S. Srivastav, N. Suzuki
This study investigated effects of chlorpyrifos on ultimobranchial (UBG) and parathyroid glands (PTG) of frog, Euphlyctis cyanophlyctis. Frogs were treated with chlorpyrifos for short and long term and sacrificed after 24, 48, 72 or 96 h in short term and after 5.10, 15 and 30 days in long term. Chlorpyrifos exposure provokes decrease in serum calcium levels after 48 h which persists till 96 h. There is slight decrease in the nuclear volume of UBG cells and cytoplasm depict weak staining response after 72 h. After 96 h these changes are more pronounced. PTG of Euphlyctis cyanophlyctis exposed to chlorpyrifos exhibit no change till 96 h. Serum calcium decreases on day 10 after chlorpyrifos exposure which continue to fall progressively till 30 days. After 15 days chloryrifos exposure, nuclear volume of UBG exhibit decrease and follicular epithelium displays decrease in height. Follicular epithelium after 30 days chlorpyrifos exposure reduces to the extent that it becomes single layered. Few degenerating cells have been discerned. At this interval nuclear volume of ultimobranchial cells exhibits a further decrease. PTG of chlorpyrifos treated frog depicts increased nuclear volume of PTG at 10 and 15 days. The nuclei of PTG are hyperchromatic and the gland becomes compact at 15 days. After 30 days following chlorpyrifos treatment nuclear volume exhibits further increase. Also degenerating cells make their appearance. Calcium regulating glands UBG and PTG of frogs were adversely affected by exposure to chlorpyrifos which may disturb the physiological functions of the organism. DOI: http://dx.doi.org/10.5281/zenodo.4004156
{"title":"Effects of chlorpyrifos on ultimobranchial and parathyroid glands of Indian skipper frog, Euphlyctis cyanophlyctis","authors":"A. Srivastav, S. Srivastava, Abhishek Kumar, S. Srivastav, N. Suzuki","doi":"10.5281/ZENODO.4004156","DOIUrl":"https://doi.org/10.5281/ZENODO.4004156","url":null,"abstract":"This study investigated effects of chlorpyrifos on ultimobranchial (UBG) and parathyroid glands (PTG) of frog, Euphlyctis cyanophlyctis. Frogs were treated with chlorpyrifos for short and long term and sacrificed after 24, 48, 72 or 96 h in short term and after 5.10, 15 and 30 days in long term. Chlorpyrifos exposure provokes decrease in serum calcium levels after 48 h which persists till 96 h. There is slight decrease in the nuclear volume of UBG cells and cytoplasm depict weak staining response after 72 h. After 96 h these changes are more pronounced. PTG of Euphlyctis cyanophlyctis exposed to chlorpyrifos exhibit no change till 96 h. Serum calcium decreases on day 10 after chlorpyrifos exposure which continue to fall progressively till 30 days. After 15 days chloryrifos exposure, nuclear volume of UBG exhibit decrease and follicular epithelium displays decrease in height. Follicular epithelium after 30 days chlorpyrifos exposure reduces to the extent that it becomes single layered. Few degenerating cells have been discerned. At this interval nuclear volume of ultimobranchial cells exhibits a further decrease. PTG of chlorpyrifos treated frog depicts increased nuclear volume of PTG at 10 and 15 days. The nuclei of PTG are hyperchromatic and the gland becomes compact at 15 days. After 30 days following chlorpyrifos treatment nuclear volume exhibits further increase. Also degenerating cells make their appearance. Calcium regulating glands UBG and PTG of frogs were adversely affected by exposure to chlorpyrifos which may disturb the physiological functions of the organism. \u0000DOI: http://dx.doi.org/10.5281/zenodo.4004156","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"26 1","pages":"296-306"},"PeriodicalIF":0.0,"publicationDate":"2020-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77533315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A new coronavirus, SARS-CoV-2, has been recognized as a cause of severe acute respiratory syndrome (SARS) and COVID-19 disease. In the absence of stable treatments for COVID-19, the possibility that vitamins: B1, C, D, and E, omega-3, minerals (magnesium and manganese), and herb thyme may have unspecified effects on infection with COVID-19 would be considered. Various reports have revealed that vitamins B1, C, D, and E, omega-3, magnesium, manganese, and thyme may affect the human innate system, for example, thiamine may play beneficial roles in human immunodeficiency viruses (HIV), treating megadose ascorbic acid can assist prevent cold and flu symptoms, vitamin D can decrease the risk of developing COVID-19, vitamin E has been evaluated against the influenza virus in mice, and omega-3 fatty acids supplementation has been efficient in reducing the severity and frequency of sickle cell rate. Magnesium may be effective in patients with a mutation in the interleukin-2-inducible T-cell kinase, as well as manganese associates with the metabolism of glucose and fats, vitamin C, and B, accelerating protein synthesis, endocrine regulation, stimulating hematopoiesis, improving innate function, and reducing reactive oxygen species (ROS) generation. Moreover, thyme extract can have beneficial antiviral effects against human papillomavirus (HPV) and influenza A (IAV). The possibility that the vitamins B1, C, D, E, omega-3, magnesium, manganese, and thyme appear to affect the human innate system warrants further study, especially in light of the recent COVID-19 epidemic. DOI: http://dx.doi.org/10.5281/zenodo.3990659
{"title":"Vitamins, omega-3, magnesium, manganese, and thyme can boost our immunity and protect against COVID-19","authors":"A. Hamada","doi":"10.5281/ZENODO.3990659","DOIUrl":"https://doi.org/10.5281/ZENODO.3990659","url":null,"abstract":"A new coronavirus, SARS-CoV-2, has been recognized as a cause of severe acute respiratory syndrome (SARS) and COVID-19 disease. In the absence of stable treatments for COVID-19, the possibility that vitamins: B1, C, D, and E, omega-3, minerals (magnesium and manganese), and herb thyme may have unspecified effects on infection with COVID-19 would be considered. Various reports have revealed that vitamins B1, C, D, and E, omega-3, magnesium, manganese, and thyme may affect the human innate system, for example, thiamine may play beneficial roles in human immunodeficiency viruses (HIV), treating megadose ascorbic acid can assist prevent cold and flu symptoms, vitamin D can decrease the risk of developing COVID-19, vitamin E has been evaluated against the influenza virus in mice, and omega-3 fatty acids supplementation has been efficient in reducing the severity and frequency of sickle cell rate. Magnesium may be effective in patients with a mutation in the interleukin-2-inducible T-cell kinase, as well as manganese associates with the metabolism of glucose and fats, vitamin C, and B, accelerating protein synthesis, endocrine regulation, stimulating hematopoiesis, improving innate function, and reducing reactive oxygen species (ROS) generation. Moreover, thyme extract can have beneficial antiviral effects against human papillomavirus (HPV) and influenza A (IAV). The possibility that the vitamins B1, C, D, E, omega-3, magnesium, manganese, and thyme appear to affect the human innate system warrants further study, especially in light of the recent COVID-19 epidemic. \u0000DOI: http://dx.doi.org/10.5281/zenodo.3990659","PeriodicalId":11771,"journal":{"name":"European Journal of Biological Research","volume":"114 1","pages":"271-295"},"PeriodicalIF":0.0,"publicationDate":"2020-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76629561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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