{"title":"Phosphate Solubilisation Potential of Screened Nitrogen Fixing Rhizobium leguminosarum Strains Isolated from Nodules of Pea Plant","authors":"P. Kaur","doi":"10.18782/2320-7051.7842","DOIUrl":null,"url":null,"abstract":"The present study was undertaken to isolate Rhizobium leguminosarum strains from the nodules of Pea (Pisum sativum (L)) var AP-3 grown in the soil of Hoshiarpur. These isolated nitrogen fixing strains were also tested for their tendency to solubilize Tricalcium Phosphate (TCP) on Pikovskaya (PVK) agar, Modified Pikovskaya (MPVK) agar and National Botanical Research Institute (NBRIP agar). The halo size of R. leguminosarum SS-8 was reported to be biggest (13 mm) on NBRIP agar and the smallest (4 mm) in PVK agar. These strains were further inoculated in PVK and NBRIP broths, respectively to see their potentials to solubilize TCP. The strain R. leguminosarum SS-8 was observed to show maximum TCP solubilisation (261 μg/ml in NBRIP and 211 μg/ml in PVK broths) with maximum decrease in pH (4.14 in NBRIP and 4.20 in PVK","PeriodicalId":14249,"journal":{"name":"International Journal of Pure & Applied Bioscience","volume":"26 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Pure & Applied Bioscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18782/2320-7051.7842","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
The present study was undertaken to isolate Rhizobium leguminosarum strains from the nodules of Pea (Pisum sativum (L)) var AP-3 grown in the soil of Hoshiarpur. These isolated nitrogen fixing strains were also tested for their tendency to solubilize Tricalcium Phosphate (TCP) on Pikovskaya (PVK) agar, Modified Pikovskaya (MPVK) agar and National Botanical Research Institute (NBRIP agar). The halo size of R. leguminosarum SS-8 was reported to be biggest (13 mm) on NBRIP agar and the smallest (4 mm) in PVK agar. These strains were further inoculated in PVK and NBRIP broths, respectively to see their potentials to solubilize TCP. The strain R. leguminosarum SS-8 was observed to show maximum TCP solubilisation (261 μg/ml in NBRIP and 211 μg/ml in PVK broths) with maximum decrease in pH (4.14 in NBRIP and 4.20 in PVK
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