Binding site and equilibrium constant of ethylenediamineplatinum(II) or triamineplatinum(II) to thymidine

Bioinorganic chemistry Pub Date : 1978-10-01 DOI:10.1016/S0006-3061(00)80027-7

Kenji Inagaki, Yoshinori Kidani

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引用次数: 4

Abstract

The complex of thymidine with (NH₃)₃Pt(OH₂) or (en)Pt(OH₂)₂, where en = ethylenediamine, have been prepared in aqueous solution at pH = 7. The isolated complexes, corresponding to the formula [(NH₃)₃Pt(ThdH₋₁)]NO₃ and (en)Pt(ThdH₋₁)₂ where ThdH₋₁ = mono anion of thymidine, have been characterized by nmr, ir, and uv spectra. The binding site is the N(3) position of ThdH₋₁.

The equilibrium constant for (NH₃)₃Pt(OH₂) + ThdH₋₁ ⇌ (NH₃)₃Pt(ThdH₋₁), in which the equilibrium of binding is pH dependent, was estimated from integrated intensity ratio of the proton of position 6 of thymidine, and log K was 10.4 ± 0.1. In the case of (en)Pt(ThdH₋₁)₂, the log K₁ for (en)Pt(OH₂)₂ + ThdH₋₁ ⇌ (en)Pt(OH₂)(ThdH₋₁) was 10.3 ± 0.1, and the log K₂ for (en)Pt(OH₂)(ThdH₋₁) + ThdH₋₁ ⇌ (en)Pt(ThdH₋₁)₂ was 7.4 ± 0.1.

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乙二胺铂(II)或三胺铂(II)与胸苷的结合位点和平衡常数

在pH = 7的水溶液中制备了胸腺嘧啶与(NH3)3Pt(OH2)或(en)Pt(OH2)2的络合物，其中en =乙二胺。分子式为[(NH3)3Pt(ThdH−1)]NO3和(en)Pt(ThdH−1)2，其中ThdH−1 =胸腺嘧啶的单阴离子，用nmr、ir和uv光谱对其进行了表征。结合位点是ThdH−1的N(3)位。根据胸腺嘧啶第6位质子的积分强度比，估计了(NH3)3Pt(OH2) + ThdH−1 + (NH3)3Pt(ThdH−1)的平衡常数，其结合平衡与pH有关，对数K为10.4±0.1。以(en)Pt(ThdH−1)2为例，(en)Pt(OH2)2 + ThdH−1 + (en)Pt(OH2)(ThdH−1)+ ThdH−1 + (en)Pt(ThdH−1)2的对数K1为10.3±0.1，(en)Pt(OH2)(ThdH−1)+ ThdH−1 + (en)Pt(ThdH−1)2的对数K2为7.4±0.1。

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Bioinorganic chemistry

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