A new sensitive spectrophotometric method for determination of vanadium has been applied to establish the vanadium concentrations in whole blood from healthy adult human males and from cancer patients in the district of Jabalpur City, India. The mean vanadium concentration in the normal blood was found to be 0.2 ppm, (range 0.18–0.22 ppm). In the cancer-patient blood the average vanadium concentration was 1.10 ppm, range 0.50–2 ppm.