The apoptotic effects of Streptozotocin in different dose and administration time on pancreatic islet cells of rats

S. Gezginci-Oktayoglu
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引用次数: 2

Abstract

The loss of islet cells by apoptotic cell death plays a central role in the pathogenesis of experimental animal models such as hyperglycemia and diabetes. Streptozotocin (STZ) is widely used for the induction of diabetes in animals by destruction of pancreatic i¢ cells. The aim of the present study is to determine the effects of different dose and administration time of STZ on pancreatic islet cells. With this aim, four different experimental groups consist of the animals treated with citrate buffer (0.01 M, pH: 4.5) only, the animals sacrificed after 5 hours of the 40 mg/kg STZ injection, the animals sacrificed after 6 hours of the 60 mg/kg STZ injection, and the animals sacrificed after 4 hours of the 75mg/kg STZ injection were composed. While the apoptotic cells were observed by TUNEL staining method and according to morphologic criteria of the cells, insulin synthesized cells were shown by immunohistochemical technique. As the result it was determined an increase in blood glucose levels and apoptotic islet cells in 40 and 75 mg/kg STZ-injected rats while islet and beta cell areas were decreased in all groups. These results indicate that 40 and 75 mg/kg STZ injection at the end of 5 and 4 hours, respectively, causes hyperglycemia by triggering apoptosis in islet cells of adult rats. Keywords: Apoptosis, hyperglycemia, islet cell, rat, streptozotocin.
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链脲佐菌素不同剂量和给药时间对大鼠胰岛细胞凋亡的影响
细胞凋亡导致的胰岛细胞损失在高血糖和糖尿病等实验动物模型的发病机制中起着核心作用。链脲佐菌素(STZ)被广泛用于通过破坏胰腺细胞诱导动物糖尿病。本研究的目的是确定STZ不同剂量和给药时间对胰岛细胞的影响。为此,实验分为4个不同的实验组:仅用柠檬酸缓冲液(0.01 M, pH: 4.5)处理的动物,40 mg/kg STZ注射5小时后死亡的动物,60 mg/kg STZ注射6小时后死亡的动物,以及75mg/kg STZ注射4小时后死亡的动物。TUNEL染色法观察凋亡细胞,根据细胞形态学标准,免疫组织化学技术显示胰岛素合成细胞。结果表明,注射40和75 mg/kg stz后,各组大鼠血糖水平升高,胰岛细胞凋亡,胰岛细胞面积减小。上述结果表明,注射40和75 mg/kg STZ分别在5和4小时后通过触发成年大鼠胰岛细胞凋亡引起高血糖。关键词:凋亡,高血糖,胰岛细胞,大鼠,链脲佐菌素。
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