Effects of ATP on Time Parameters of Contractility of Rats’ Slow and Fast Skeletal Muscles in Normal and Hypothermic Conditions

IF 0.5 Q4 ORTHOPEDICS MLTJ-Muscles Ligaments and Tendons Journal Pub Date : 2023-01-12 DOI:10.3390/muscles2010003
A. E. Khairullin, S. Grishin, Azat I. Gabdrahmanov, A. U. Ziganshin
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Abstract

We have previously shown that hypothermia leads to an increase in the synaptic modulating effects of ATP but not of adenosine in several different animal skeletal muscles. In this paper, we studied the effect of ATP on the amplitude–time parameters of single and tetanic contractions of rats’ isolated fast (1) and slow (2) muscles at different temperatures. We found that when muscles were stimulated by the electrical field (0.1 Hz, 0.5 ms, 10 V), with a decrease in the bath temperature from 37 °C to 14 °C (3), there was an increase in the half-relaxation time of the slow muscle (m. soleus), but not of the fast muscle (m. EDL). Similar effects were observed using a carbachol-induced contraction technique, which suggests the postsynaptic (4) nature of the expansion of the contractile response of the slow muscle induced by ATP (5). To confirm the postsynaptic nature of the observed phenomenon, experiments were performed at a high calcium level (7.2 mM), in which the presynaptic effects of ATP were shown to be offset. We found that the hypercalcium condition did not significantly change the effects of ATP on the measured parameters in both muscles. To record muscle tetanic contractions, we gradually increased the frequency of electrical impulses with the increment of 2.5 Hz to achieve the fusion frequencies of 12.5 Hz for m. soleus and 17.5 Hz for m. EDL at normal temperatures. ATP (100 μM) did not change the fusion frequency for both muscles at a normal temperature but decreased this parameter for the slow muscle to 5 Hz at 14 °C without affecting that for the fast muscle. We conclude that ATP potentiates a hypothermia-induced increase in the half-relaxation time of the contraction of rats’ slow, but not fast, skeletal muscles by acting on postsynaptic P2 receptors (6).
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ATP对正常和低温条件下大鼠慢速和快速骨骼肌收缩时间参数的影响
我们之前已经证明,低温导致几种不同动物骨骼肌中ATP的突触调节作用增加,而腺苷的突触调节作用没有增加。本文研究了ATP对大鼠离体快肌(1)和慢肌(2)在不同温度下单次和强直收缩幅度-时间参数的影响。我们发现,当肌肉受到电场(0.1 Hz, 0.5 ms, 10 V)刺激时,随着浴温从37°C降低到14°C(3),慢肌(m.比目鱼肌)的半松弛时间增加,而快肌(m. EDL)的半松弛时间没有增加。使用碳水化合物诱导的收缩技术观察到类似的效果,这表明ATP诱导的慢肌收缩反应扩张的突触后(4)性质(5)。为了证实所观察到的现象的突触后性质,实验在高钙水平(7.2 mM)下进行,其中ATP的突触前效应被证明是抵消的。我们发现高钙状态并没有显著改变ATP对两个肌肉测量参数的影响。为了记录肌肉强直收缩,我们逐渐增加电脉冲频率,增加2.5 Hz,达到常温下比目鱼肌12.5 Hz和EDL肌17.5 Hz的融合频率。ATP (100 μM)在常温下没有改变两种肌肉的融合频率,但在14℃下将慢肌的融合频率降低到5 Hz,但不影响快肌的融合频率。我们得出结论,ATP通过作用于突触后P2受体,增强了低温诱导的大鼠缓慢而非快速的骨骼肌收缩半松弛时间的增加(6)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
51
期刊介绍: MLTJ (Muscle, Ligaments and Tendons Journal) is an open access, peer-reviewed online journal that encompasses all aspects of clinical and basic research studies related to musculoskeletal, ligament, tendon, public health, exercise physiology and kinesiology issues. Muscle, Ligaments and Tendons Journal (MLTJ) provides the platform for exchange of new clinical and scientific information in the most precise and expeditious way to achieve timely dissemination of information and cross-fertilization of ideas. It is the official journal of the Italian Society of Muscles, Ligaments and Tendons (I.S.Mu.L.T.), Società Italiana Terapia con Onde D’urto (S.I.T.O.D.) and Società Italiana Studio Piede e Caviglia (S.I.S.P.E.C)
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