Comparison of genetic characteristics among species of Suaeda spp. (Amaranthaceae) in the western Anbar plateau - Iraq

S. Alrawi, H. Buniya, N. Al-abide
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Abstract

Background: Comparison of genetic characteristics among species of Suaeda spp. Objective: identifying the genetic characteristics among species Suaeda (Amaranthaceae) in Anbar Governorate. (From August 2020 to September 2021  fresh plant samples were collected in the flowering stage for the studied species). Materials and methods: The genetic diversity of the Suaeda species was studied after DNA extraction and using the Inter Simple Sequence Repeats Reaction method to recorded in the genetic aspects. DNA was extracted from young leaves of the studied species. The concentration and purity of the DNA were determined, and the detection of genetic relationships between the studied plant species as well as finding this genetic fingerprint by ISSR Reaction. Results: The results showed that the plant samples collected during study belong to species of the genus Suaeda, and these species were: S. aegyptiaca, S. altissima, S. carnosissma, S. fruticosa, S. monoica, S. vera and S. vermiculata symbolized by (S1, S2, S3, S4, S5, S6, S7) respectively for the purpose of brevity. The results showed that there was the highest close between S. aegyptiaca and S. vera which amounted to 0.6319, and the results showed that there was the least closeness between S. aegyptiaca and S. carnosissma which amounted to 0.4231. Conclusion: Genetic traits are among the stable traits that can be adopted in separating the studied species. The genetic study, especially at the DNA level, is one of the most important modern taxonomic studies that rely on PCR technology for the accuracy of its results and the speed of obtaining results.There are at least seven species of the genus Suaeda in the western province of Anbar Governorate - Iraq.
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伊拉克安巴尔高原西部苋属植物遗传特征比较
背景:紫苏属植物种间遗传特征比较。目的:鉴定安巴尔省紫苏属植物的遗传特征。(从2020年8月到2021年9月,在研究物种的开花阶段收集了新鲜植物样本)。材料与方法:提取Suaeda属植物的DNA,利用Inter Simple Sequence Repeats Reaction法进行遗传多样性研究。从研究物种的嫩叶中提取DNA。测定DNA的浓度和纯度,利用ISSR反应检测所研究植物物种间的亲缘关系,并寻找该遗传指纹。结果:本研究所采集的植物标本均为水韭属植物,为简便,分别为埃及水韭、高原水韭、肉韭、果韭、单叶水韭、真丝水韭和水蛭水韭,分别用(S1、S2、S3、S4、S5、S6、S7)表示。结果表明,埃及伊蚊与维拉伊蚊的接近度最高,为0.6319;埃及伊蚊与肉毒杆菌的接近度最低,为0.4231。结论:遗传性状是可用于物种分离的稳定性状之一。遗传研究,特别是DNA水平的遗传研究,是现代分类学研究中最重要的领域之一,其结果的准确性和获得结果的速度依赖于PCR技术。在伊拉克西部的安巴尔省,至少有7种Suaeda属植物。
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