Antibacterial and hemolytic activities of different extracts of Amsonia orientalis Decne (Apocynaceae)

A. Erdem, E. Ö. A. Aydoğdu, G. Öz, Ç. Erol, M. Yazgan
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引用次数: 1

Abstract

In the current study, the antibacterial activities of the crude extracts (leaf and stem chloroform and methanol; leaf hexane, acetone, deionized water and ethanol; stem deionized water) of Amsonia orientalis Decne. (Blue Star) were investigated against 17 different strains of Gram-negative and Gram-positive bacteria. To evaluate the antibacterial and hemolitic activities of extracts standard antibiotic discs and human erythrocytes were used, respectively. The antibacterial activity was determed in the all extracts, except the hexane extracts of the leaves. It was observed that Bacillus subtilis ATCC 6633, Legionella pneumophila ATCC 33152 and L. pneumophila serogroup (SG) 2-14 are the most susceptible bacteria to the different extracts, while Pseudomonas aeruginosa ATCC 9327, Staphylococcus aureus ATCC 6538, Enterococcus faecalis ATCC 10541 and Proteus mirabilis are resistant bacteria. It was determined that chloroform (leaf and stem), hexane (leaf) and deionized water (stem) extracts did not show hemolytic activity, while ethanol (leaf) (≥5 mg/ml), methanol (stem and leaf) (≥10 mg/ml), acetone (leaf) (≥10 mg/ml) and deionized water (leaf) (≥20 mg/ml) extracts showed hemolytic activity. Key words: Amsonia orientalis, Antibacterial activity, Hemolytic activity, Plant extract. *Corresponding Author: Elif Ozlem ARSLAN AYDOĞDU (e-mail: eoarslan@istanbul.edu.tr ) (Received: 01.03.2012 Accepted: 21.09.2012)
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夹竹桃科木槐不同提取物的抗菌和溶血活性研究
在本研究中,粗提物(叶、茎、氯仿、甲醇;叶己烷、丙酮、去离子水和乙醇;干去离子水)的Amsonia orientalis Decne。对17株革兰氏阴性菌和革兰氏阳性菌进行检测。分别用标准抗生素片和人红细胞评价其抑菌和溶血活性。对除己烷提取物外的所有提取物进行抑菌活性测定。结果显示,枯草芽孢杆菌ATCC 6633、嗜肺军团菌ATCC 33152和嗜肺乳杆菌血清群(SG) 2-14对不同提取物最敏感,铜绿假单胞菌ATCC 9327、金黄色葡萄球菌ATCC 6538、粪肠球菌ATCC 10541和神奇变形杆菌为耐药菌。结果表明,氯仿(叶和茎)、己烷(叶)和去离子水(茎)提取物不具有溶血活性,乙醇(叶)(‰5 mg/ml)、甲醇(茎和叶)(‰10 mg/ml)、丙酮(叶)(‰10 mg/ml)和去离子水(叶)(‰20 mg/ml)提取物具有溶血活性。关键词:东方氨,抗菌活性,溶血活性,植物提取物*通讯作者:Elif Ozlem ARSLAN AYDOĞDU (e-mail: eoarslan@istanbul.edu.tr)(收稿日期:01.03.2012,收稿日期:21.09.2012)
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