Binding of the Bi (III) Complex of Naringin with β-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics

Abstract

Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb with β-Cyclodextrin (β-CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb with β-CD was 1 : 1 with a binding constant of 5.18 × 102 mol−1 dm3. The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence of β-CD. The binding constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.29 × 105 mol−1 dm3 and 6.89 × 104 mol−1 dm3, respectively. The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.25 × 104 mol−1 dm3 and 5.10 × 103 mol−1 dm3, respectively. The lowering of the binding affinity and the were observed for the interaction of Narb with ctDNA in the presence of β-CD.