Evaluation of BRAF Gene Status in Gliomas

Neurology and Neurobiology Pub Date : 2021-08-16 DOI:10.31487/j.nnb.2021.03.02

A. Jaiswal, S. Agrawal, S. Jaiswal, K. Das, S. Behari, S. Tewari, Madam Mohan Godbole, P. Misra

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Abstract

Background: Development of different molecular markers has given a new insight in the glioma management. KIAA1549-BRAF gene fusion has a diagnostic and prognostic significance. Aim: The aim of this study was to determine the KIAA1549-BRAF gene fusion in glioma and their correlation with various clinical parameters. Material and Methods: Forty cases of glioma were studied for KIAA1549-BRAF gene fusion by reverse transcription-PCR (RT-PCR). Results: Overall, KIAA1549-BRAF gene fusion was found in 22% (9/40) cases of glioma. Children had higher KIAA1549-BRAF fusion (72%; 8/11) as compared to adults (10%; 3/29) and this difference was statistically significant. Cerebellar location of tumor was significantly associated with KIAA1549-BRAF fusion. KIAA1549-BRAF fusion was highest in pilocytic astrocytoma (89%), and this difference was statistically significant. Statistically significant difference was noted between KIAA1549-BRAF fusion expression and WHO grade I glioma. Conclusion: Overall, KIAA1549-BRAF gene fusion was found in 22% (9/40) cases of glioma. Childhood age, pilocytic astrocytoma histology, cerebellar location and WHO grade I tumor were significantly associated with KIAA1549-BRAF gene fusion.

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脑胶质瘤中BRAF基因状态的评估

背景:不同分子标记的发展为胶质瘤的治疗提供了新的视角。KIAA1549-BRAF基因融合具有诊断和预后意义。目的:本研究的目的是确定KIAA1549-BRAF基因在胶质瘤中的融合及其与各种临床参数的相关性。材料与方法:采用逆转录- pcr (RT-PCR)对40例胶质瘤患者进行KIAA1549-BRAF基因融合研究。结果:总体而言，在22%(9/40)的胶质瘤病例中发现KIAA1549-BRAF基因融合。儿童KIAA1549-BRAF融合率较高(72%;8/11)，与成人相比(10%;3/29)，差异有统计学意义。小脑肿瘤位置与KIAA1549-BRAFfusion显著相关。KIAA1549-BRAF融合在毛细胞星形细胞瘤中最高(89%)，差异有统计学意义。KIAA1549-BRAF融合表达与WHO一级胶质瘤差异有统计学意义。结论:总体而言，在22%(9/40)的胶质瘤病例中发现KIAA1549-BRAF基因融合。儿童年龄、毛细胞星形细胞瘤组织学、小脑位置和WHO一级肿瘤与KIAA1549-BRAF基因融合显著相关。

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Neurology and Neurobiology

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