Adenosine deaminase (ADA) activity and isozymes in the serum of patients with hepatitis B compared with healthy people: a useful method in diagnosis clinical status

S. Athari
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引用次数: 1

Abstract

Background: Adenosine deaminase (ADA) specifications (EC3.5.4.4) is an enzyme is involved in purine metabolism that breaks adenosine and deoxy-adenosine and produce inosine and deoxy-inosine and ammonia. The highest levels are of adenosine deaminase activity in monocytes and lymphocytes. High serum ADA activity with increased serum levels of AST, ALT and immunoglobulins were reported in variety of diseases including hepatitis. We aimed to investigate the activity of total ADA in serum of patients with hepatitis B and were determined molecular weight ADA1 and ADA2 isozymes in serum and RBC of hepatitis patients. Materials and Methods: We were defining experiments by electrophoresis on SDS-PAGE, for isozymes of ADA; ADA1 and ADA2 in serum and red blood cells. ADA1 molecular weight was estimated at about 35 KDa and ADA2 about 110 KDa. The total ADA activity was measured by the modified Ellis method in 37 patients with hepatitis B and 40 healthy controls in the age range (20-60 years). Results: The normal values for serum ADA in humans have been studied by various workers and found in serum samples to be in the range of 0-15 U/L, whiles in our analysis total ADA (tADA) enzyme activity is in controls and patients with hepatitis B, respectively, 13.35 ± 1.62 and 27.05 ± 8.49. Our results indicated that tADA level was higher in patients with hepatitis B than those of corresponding controls (P < 0.05).Total ADA enzyme activity shows a significant increase compared to the control group in all age groups tested. Conclusion: Therefore, the serum ADA level could be used as an index along with other parameters in follow up of patients with hepatitis B.
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乙型肝炎患者血清腺苷脱氨酶(ADA)活性及同工酶与健康人的比较:一种诊断临床状况的有用方法
背景:腺苷脱氨酶(Adenosine deaminase, ADA)规范(EC3.5.4.4)是一种参与嘌呤代谢的酶,可分解腺苷和脱氧腺苷,产生肌苷和脱氧肌苷和氨。单核细胞和淋巴细胞的腺苷脱氨酶活性最高。据报道,在包括肝炎在内的多种疾病中,血清ADA活性高且血清AST、ALT和免疫球蛋白水平升高。目的探讨乙型肝炎患者血清中ADA1和ADA2同工酶的活性,并测定肝炎患者血清和红细胞中ADA1和ADA2同工酶的分子量。材料与方法:采用SDS-PAGE电泳法测定ADA同工酶;血清和红细胞中ADA1和ADA2。ADA1分子量约为35 KDa, ADA2分子量约为110 KDa。采用改良的Ellis方法测定37例乙型肝炎患者和40例年龄在20-60岁的健康对照者的总ADA活性。结果:不同工作人员对人类血清ADA的正常值进行了研究,发现血清样品中ADA (tADA)酶活性在0-15 U/L范围内,而在我们的分析中,对照组和乙型肝炎患者的总ADA (tADA)酶活性分别为13.35±1.62和27.05±8.49。结果显示,乙型肝炎患者tADA水平明显高于对照组(P < 0.05)。与对照组相比,所有年龄组的ADA酶活性都有显著增加。结论:血清ADA水平可与其他指标一起作为乙型肝炎患者随访的指标。
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