Zeynab Faraji, Behnoush Khasheii, E. Ghaemi, Shaghayegh Anvari, R. Rajabnia, A. Jamali
Background and Aim: Urinary tract infections (UTIs) are one of the most common pathological diseases in communities and hospitals, often caused by uropathogenic Escherichia coli (UPEC). The use of microbial strains typing is an integral part of epidemiological surveys of infectious diseases to identify epidemics, detect the infection source, track and recognize pathogenic strains. In this study, uropathogenic Escherichia coli (UPEC) strains isolated from patients living in Gorgan were typed using RAPD-PCR. Methods: In total, 187 Escherichia coli strains isolated from urine samples of inpatients and outpatients of Gorgan city from 2010 to 2016 were analyzed by the RAPD-PCR method using two primers. Using GelClust and FigTree software, the respective dendrograms were plotted by unweighted pair group method with arithmetic mean (UPGMA). Results: In our research, 614 bands were detected using two primers. The highest frequency of bands was obtained in 400 bp and 500 bp with 65 repeats and the lowest number of bands was in 2500 bp and 3000 bp with one repeat and 32 clusters. The largest number of isolates (i.e.14) was placed in cluster 16. Most bands were polymorphic, indicating high genetic diversity in isolates. Conclusion: Analysis of 32 clusters of our study by the RAPD-PCR method showed that the studied clusters do not have a specific and unique feature and the scattering of isolates properties are equal among the clusters. Because each cluster had its characteristics, E. coli strains in the region have great genetic diversity. *Corresponding Author: Ailar Jamali; Email: jamali@goums.ac.ir; ORCID: https://orcid.org/0000-0002-4612-8144 Please cite this article as: Faraji Z, Khasheii B, Ghaemi EA, Anvari S, Rajabnia R, Jamali A. Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients in Gorgan by Random Amplified Polymorphic DNA-PCR (RAPD-PCR). Arch Med Lab Sci. 2021;7:1-7 (e17). https://doi.org/10.22037/amls.v7.34383
{"title":"Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients in Gorgan by Random Amplified Polymorphic DNA-PCR (RAPD-PCR)","authors":"Zeynab Faraji, Behnoush Khasheii, E. Ghaemi, Shaghayegh Anvari, R. Rajabnia, A. Jamali","doi":"10.22037/AMLS.V7.34383","DOIUrl":"https://doi.org/10.22037/AMLS.V7.34383","url":null,"abstract":"Background and Aim: Urinary tract infections (UTIs) are one of the most common pathological diseases in communities and hospitals, often caused by uropathogenic Escherichia coli (UPEC). The use of microbial strains typing is an integral part of epidemiological surveys of infectious diseases to identify epidemics, detect the infection source, track and recognize pathogenic strains. In this study, uropathogenic Escherichia coli (UPEC) strains isolated from patients living in Gorgan were typed using RAPD-PCR. \u0000Methods: In total, 187 Escherichia coli strains isolated from urine samples of inpatients and outpatients of Gorgan city from 2010 to 2016 were analyzed by the RAPD-PCR method using two primers. Using GelClust and FigTree software, the respective dendrograms were plotted by unweighted pair group method with arithmetic mean (UPGMA). \u0000Results: In our research, 614 bands were detected using two primers. The highest frequency of bands was obtained in 400 bp and 500 bp with 65 repeats and the lowest number of bands was in 2500 bp and 3000 bp with one repeat and 32 clusters. The largest number of isolates (i.e.14) was placed in cluster 16. Most bands were polymorphic, indicating high genetic diversity in isolates. \u0000Conclusion: Analysis of 32 clusters of our study by the RAPD-PCR method showed that the studied clusters do not have a specific and unique feature and the scattering of isolates properties are equal among the clusters. Because each cluster had its characteristics, E. coli strains in the region have great genetic diversity. \u0000*Corresponding Author: Ailar Jamali; Email: jamali@goums.ac.ir; ORCID: https://orcid.org/0000-0002-4612-8144 \u0000Please cite this article as: Faraji Z, Khasheii B, Ghaemi EA, Anvari S, Rajabnia R, Jamali A. Molecular Typing of Uropathogenic Escherichia coli Strains Isolated from Patients in Gorgan by Random Amplified Polymorphic DNA-PCR (RAPD-PCR). Arch Med Lab Sci. 2021;7:1-7 (e17). https://doi.org/10.22037/amls.v7.34383","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80180821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Torabizadeh, V. Dana, S. A. Aghapour, K. Zibara, Z. Zayeri, Elham Rajaei
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses several molecules such as angiotensin-converting enzyme 2 (ACE2), cluster of differentiation 26 (CD26), Ezrin, and Neuropilin-1 (NRP-1) for viral entry. In this review, the entire structural and genomic combination and the mechanism of virus entry, are discussed. This study might be useful for further drug design studies. SARS-CoV-2 neutralization allows the immune system to fight the virus before its entry. COVID-19 enters the host bloodstream by infecting endothelial cells via a cluster of differentiation 147 (CD147). SARS-CoV-2 not only uses ACE2 for its entry but also affects ACE-2 and its enzymatic activity on Ang II and bradykinin, it also imbalances the RAAS and bradykinin system and elevates the inflammation. High levels of bradykinin, cause nonproductive cough as the result of fluid extravasation and leukocyte recruitment to the lung. Accordingly, we suggest replicase transcriptase complex (RTC) and specific non-structural proteins (Nsps) such as Nsp7,8, Nsp10, Nsp12, and Nsp16 are perfect targets of study because RTC and Nsps are the golden elements in the maintenance of COVID-19 appearance and masking. Base on this evidence COVID-19 uses various receptors for its entry and it might block these receptors' activity to evade the immune system and spread to other cells. *Corresponding Authors: Elham Rajaei, Email: elhamrj@gmail.com; ORCID: https://orcid.org/0000-0002-8231-0138 Please cite this article as: Torabizadeh M, Ghobadi Dana V, Aghapour SA, Zibara K, Deris Zayeri Z, Rajaei E. A Review of SARS-CoV-2 Genetic and Structure: Hot Cellular Targets for Virus Entry. Arch Med Lab Sci. 2021;7:1-9 (e15). https://doi.org/10.22037/amls.v7.34216
严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)利用血管紧张素转换酶2 (ACE2)、分化簇26 (CD26)、Ezrin和神经匹林-1 (NRP-1)等分子进入病毒。本文综述了病毒的整体结构和基因组组合以及病毒进入的机制。本研究可能为进一步的药物设计研究提供参考。SARS-CoV-2的中和使免疫系统能够在病毒进入之前与之对抗。COVID-19通过分化簇147 (CD147)感染内皮细胞进入宿主血液。SARS-CoV-2不仅利用ACE2进入，还影响ACE2及其对Ang II和缓激肽的酶活性，使RAAS和缓激肽系统失衡，加重炎症反应。高水平的缓激肽，由于液体外溢和白细胞聚集到肺部而引起非生产性咳嗽。因此，我们认为复制酶转录酶复合体(RTC)和特异性非结构蛋白(Nsps)如Nsp7、8、Nsp10、Nsp12和Nsp16是理想的研究目标，因为RTC和Nsps是维持COVID-19外观和掩蔽的黄金元素。基于这一证据，COVID-19使用各种受体进入，它可能会阻止这些受体的活性，以逃避免疫系统并扩散到其他细胞。*通讯作者:Elham Rajaei, Email: elhamrj@gmail.com;ORCID: https://orcid.org/0000-0002-8231-0138本文全文如下:Torabizadeh M, Ghobadi Dana V, Aghapour SA, Zibara K, Deris Zayeri Z, Rajaei E. SARS-CoV-2基因和结构综述:病毒进入的热细胞靶点。中华医学杂志，2021;7:1-9 (e15)。https://doi.org/10.22037/amls.v7.34216
{"title":"A Review of SARS-CoV-2 Genetic and Structure: Hot Cellular Targets for Virus Entry:","authors":"M. Torabizadeh, V. Dana, S. A. Aghapour, K. Zibara, Z. Zayeri, Elham Rajaei","doi":"10.22037/AMLS.V7.34216","DOIUrl":"https://doi.org/10.22037/AMLS.V7.34216","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses several molecules such as angiotensin-converting enzyme 2 (ACE2), cluster of differentiation 26 (CD26), Ezrin, and Neuropilin-1 (NRP-1) for viral entry. In this review, the entire structural and genomic combination and the mechanism of virus entry, are discussed. This study might be useful for further drug design studies. SARS-CoV-2 neutralization allows the immune system to fight the virus before its entry. COVID-19 enters the host bloodstream by infecting endothelial cells via a cluster of differentiation 147 (CD147). SARS-CoV-2 not only uses ACE2 for its entry but also affects ACE-2 and its enzymatic activity on Ang II and bradykinin, it also imbalances the RAAS and bradykinin system and elevates the inflammation. High levels of bradykinin, cause nonproductive cough as the result of fluid extravasation and leukocyte recruitment to the lung. Accordingly, we suggest replicase transcriptase complex (RTC) and specific non-structural proteins (Nsps) such as Nsp7,8, Nsp10, Nsp12, and Nsp16 are perfect targets of study because RTC and Nsps are the golden elements in the maintenance of COVID-19 appearance and masking. Base on this evidence COVID-19 uses various receptors for its entry and it might block these receptors' activity to evade the immune system and spread to other cells. \u0000*Corresponding Authors: Elham Rajaei, Email: elhamrj@gmail.com; ORCID: https://orcid.org/0000-0002-8231-0138 \u0000Please cite this article as: Torabizadeh M, Ghobadi Dana V, Aghapour SA, Zibara K, Deris Zayeri Z, Rajaei E. A Review of SARS-CoV-2 Genetic and Structure: Hot Cellular Targets for Virus Entry. Arch Med Lab Sci. 2021;7:1-9 (e15). https://doi.org/10.22037/amls.v7.34216","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85440441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Barati, S. Mousavi, Jamal Motallebzadeh Khanmiri, Mohammad Khani-Eshratabdi
Background and Aim: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells affecting both children and adults. Long non-coding RNAs (lncRNAs) are one kind of non-coding RNAs (ncRNAs), reported modulating the initiation or progression of diverse cancers. However, the role of CCDC26 and FOXCUT long non-coding RNAs in ALL has been unknown. In this study, we explored the expression of FOXCUT and CCDC26 lncRNAs in acute lymphoblastic leukemia cell lines. Methods: Acute T lymphoblastic leukemia cell lines, RPMI 8402, Jurkat, B lymphoblastic leukemia, Daudi, and Ramos cell lines were used. After culturing the cells, RNA extraction and cDNA synthesis were performed. The real-time PCR technique was then used to study the expression of CCDC26, FOXCUT, C-kit, and FOXC1 genes. Result: We found a significant increase of CCDC26 expression in RPMI 8402 (p <0.0001) and Ramos (p <0.05) cell lines compared to the control, while decreased expression of these genes was observed in Jurkat and Daudi cell lines. Furthermore, FOXCUT gene had a significant increase in expression in all cell lines compared to the control (p <0.01 in Daudi and RPMI 8402 cell lines) (p <0.001 in Jurkat and Ramos cell lines). Conclusion: Our results demonstrated that CCDC26 and FOXCUT genes can play a regulatory role in acute lymphoblastic leukemia and may serve as a potential diagnostic biomarker and therapeutic target of acute lymphoblastic leukemia. *Corresponding Author: Seyed Hadi Mousavi, Email: hmousavi@tums.ac.ir; ORCID: https://orcid.org/0000-0002-4686-5232 Please cite this article as: Barati S, Mousavi SH, Motallebzadeh-Khanmiri J, Khani-Eshratabdi M. The Expression Level of CCDC26 and FOXCUT Genes in Acute Lymphoblastic Leukemia. Arch Med Lab Sci. 2021;7:1-9 (e16). https://doi.org/10.22037/amls.v7.33294
{"title":"The Expression Level of CCDC26 and FOXCUT Genes in Acute Lymphoblastic Leukemia","authors":"S. Barati, S. Mousavi, Jamal Motallebzadeh Khanmiri, Mohammad Khani-Eshratabdi","doi":"10.22037/AMLS.V7.33294","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33294","url":null,"abstract":"Background and Aim: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells affecting both children and adults. Long non-coding RNAs (lncRNAs) are one kind of non-coding RNAs (ncRNAs), reported modulating the initiation or progression of diverse cancers. However, the role of CCDC26 and FOXCUT long non-coding RNAs in ALL has been unknown. In this study, we explored the expression of FOXCUT and CCDC26 lncRNAs in acute lymphoblastic leukemia cell lines. \u0000Methods: Acute T lymphoblastic leukemia cell lines, RPMI 8402, Jurkat, B lymphoblastic leukemia, Daudi, and Ramos cell lines were used. After culturing the cells, RNA extraction and cDNA synthesis were performed. The real-time PCR technique was then used to study the expression of CCDC26, FOXCUT, C-kit, and FOXC1 genes. \u0000Result: We found a significant increase of CCDC26 expression in RPMI 8402 (p <0.0001) and Ramos (p <0.05) cell lines compared to the control, while decreased expression of these genes was observed in Jurkat and Daudi cell lines. Furthermore, FOXCUT gene had a significant increase in expression in all cell lines compared to the control (p <0.01 in Daudi and RPMI 8402 cell lines) (p <0.001 in Jurkat and Ramos cell lines). \u0000Conclusion: Our results demonstrated that CCDC26 and FOXCUT genes can play a regulatory role in acute lymphoblastic leukemia and may serve as a potential diagnostic biomarker and therapeutic target of acute lymphoblastic leukemia. \u0000*Corresponding Author: Seyed Hadi Mousavi, Email: hmousavi@tums.ac.ir; \u0000ORCID: https://orcid.org/0000-0002-4686-5232 \u0000Please cite this article as: Barati S, Mousavi SH, Motallebzadeh-Khanmiri J, Khani-Eshratabdi M. The Expression Level of CCDC26 and FOXCUT Genes in Acute Lymphoblastic Leukemia. Arch Med Lab Sci. 2021;7:1-9 (e16). https://doi.org/10.22037/amls.v7.33294","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82441341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Mehrpour, M. Dastjerdi, Samaneh Nakhaee, Alireza Amirabadizadeh, B. Bijari, Hesam Roumi, Mehran Hosseini
Background and Aim: Acetaminophen (APAP) is a commonly used analgesic and also the leading cause of medication-induced liver damage. On the other hand, N-acetylcysteine (NAC) is a medication widely used to treat APAP overdose. Despite this interest, a few studies have investigated the co-administration effects of these medications. Therefore, this study aimed to evaluate the effects of NAC and APAP on renal and liver functions in rats when they use concurrently. Methods: Male Wistar rats were orally treated with a single dose of APAP (700 mg/kg) alone or in combination of NAC at the three different doses (200, 500, and 700 mg/kg). After 24 hours, the blood and liver samples were collected for biochemical and histopathological evaluations. Results: Liver damage was well established in the 700 mg/kg APAP-treated rats, as evidenced by elevated the plasma levels of aspartate transaminase (AST) and alanine transaminase (ALT). In addition, the plasma level of blood urea nitrogen (BUN) was significantly increased in the APPA group compared to the control group. Moreover, histological examinations revealed that liver degeneration was evident in APAP-treated animals. NAC only at the highest dose (700 mg/kg) could inhibit ALT elevation, but had no effect on AST and BUN levels. Interestingly, co-administration of NAC (700 mg/kg) with APAP (700 mg/kg) could slightly shift liver histological alterations from the irreversible stage (fibrosis) toward reversible lesions such as necrosis and hemorrhage. Conclusion: The study findings indicate that co-administration of NAC and APAP can reduce the severity of APAP-induced liver damage in rats. *Corresponding Author: Mehran Hosseini; Email: mehranhosseiny@bums.ac.ir; ORCID: https://orcid.org/0000-0002-6793-2035 Please cite this article as: Mehrpour O, Dastjerdi M, Nakhaee S, Amirabadizadeh A, Bijari B, Roomi H, Hosseini M. Formulating a New Pharmaceutical Drug; Acetaminophen Tablet Containing N-acetyl Cysteine, To Alleviate the Severity of Liver Damage in Rats: Phase I, Animal Study. Arch Med Lab Sci. 2021;7:1-8 (e14). https://doi.org/10.22037/amls.v7.35528
背景与目的:对乙酰氨基酚(APAP)是一种常用的镇痛药，也是引起药物性肝损伤的主要原因。另一方面，n -乙酰半胱氨酸(NAC)是一种广泛用于治疗APAP过量的药物。尽管有这种兴趣，一些研究调查了这些药物的共同施用效果。因此，本研究旨在评价NAC和APAP同时使用时对大鼠肝肾功能的影响。方法:雄性Wistar大鼠口服单剂量APAP (700 mg/kg)或NAC(200、500、700 mg/kg)联合治疗。24小时后，采集血液和肝脏标本进行生化和组织病理学评价。结果:700 mg/kg apap处理大鼠肝损伤明显，血浆中谷草转氨酶(AST)和丙氨酸转氨酶(ALT)水平升高。此外，与对照组相比，APPA组血浆尿素氮(BUN)水平显著升高。此外，组织学检查显示，apap处理的动物肝脏变性明显。NAC仅在最高剂量(700 mg/kg)时能抑制ALT升高，但对AST和BUN水平无影响。有趣的是，NAC (700 mg/kg)和APAP (700 mg/kg)联合给药可以轻微地将肝脏组织学改变从不可逆阶段(纤维化)转变为可逆性病变，如坏死和出血。结论:NAC与APAP联合用药可减轻APAP所致大鼠肝损伤的严重程度。*通讯作者:Mehran Hosseini;电子邮件:mehranhosseiny@bums.ac.ir;ORCID: https://orcid.org/0000-0002-6793-2035本文署名:Mehrpour O, Dastjerdi M, Nakhaee S, Amirabadizadeh A, Bijari B, Roomi H, Hosseini M.新药配方;含n -乙酰半胱氨酸对乙酰氨基酚片减轻大鼠肝损伤的ⅰ期动物研究中华医学杂志，2011;7:1-8 (e14)。https://doi.org/10.22037/amls.v7.35528
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Morteza Shakeri Hoseinabad, E. Aryan, M. Mobarhan, M. Moohebati, Samaneh Abolbashari, Aida Gholoobi, Amin Houshyar Chechaklou, A. Yaghoubi, M. Meshkat, Z. Meshkat
Background and Aim: Hepatitis C virus (HCV) infection is one of the leading causes of morbidity and mortality worldwide. It has been hypothesized that a number of bacteria and viruses might be involved in the pathogenesis of cardiovascular disease. The aim of this study was to define the prevalence of HCV in patients with cardiovascular disease in comparison with a control group. Methods: In this study, 281 individuals including 143 cardiovascular patients and 138 healthy controls were assessed for identification of HCV antibodies. The data collection was done between April 2016 and February 2017. The prevalence of HCV antibodies was determined by the enzyme-linked immunosorbent assay (ELISA) method. Results: There was no HCV infection in both patients with or without cardiovascular disease. There was a significant direct correlation between cardiovascular diseases and mean level of FPG (Fasting plasma glucose) (p<0.001). Also the Systolic and Diastolic blood pressures were significantly higher in the patients with cardiovascular disease (p<0.001 and p=0.005, respectively). Conclusion: The results of this study show that no evidence of HCV infection is found among a group of cardiovascular patients in the city of Mashhad. *Corresponding Author: Zahra Meshkat; Email: meshkatz@mums.ac.ir Please cite this article as: Shakeri Hoseinabad M, Aryan E, Ghayour Mobarhan M, Moohebati M, Abolbashari S, Gholoobi A, Houshyar Chechaklou A, Yaghoubi A, Meshkat M, Meshkat Z. No Evidence of Hepatitis C Virus Infection in Individuals with Cardiovascular Disease in Mashhad. Arch Med Lab Sci. 2021;7:1-5 (e13). https://doi.org/10.22037/amls.v7.33445
背景与目的:丙型肝炎病毒(HCV)感染是全球发病率和死亡率的主要原因之一。据推测，许多细菌和病毒可能参与心血管疾病的发病机制。本研究的目的是确定HCV在心血管疾病患者中的患病率，并与对照组进行比较。方法:本研究对281例患者(包括143例心血管患者和138例健康对照)进行HCV抗体检测。数据收集于2016年4月至2017年2月期间完成。采用酶联免疫吸附试验(ELISA)测定HCV抗体的流行率。结果:不论有无心血管疾病，患者均无HCV感染。空腹血糖(FPG)与心血管疾病有显著的直接相关性(p<0.001)。心血管疾病患者的收缩压和舒张压也明显升高(p<0.001和p=0.005)。结论:本研究结果表明，在马什哈德市的一组心血管患者中没有发现HCV感染的证据。*通讯作者:Zahra Meshkat;Shakeri Hoseinabad M, Aryan E, Ghayour Mobarhan M, Moohebati M, Abolbashari S, Gholoobi A, Houshyar Chechaklou A, Yaghoubi A, Meshkat M, Meshkat Z.马什哈德心血管疾病患者丙型肝炎病毒感染的证据中华医学杂志，2021;7:1-5 (e13)。https://doi.org/10.22037/amls.v7.33445
{"title":"No Evidence of Hepatitis C Virus Infection in Individuals with Cardiovascular Disease in Mashhad","authors":"Morteza Shakeri Hoseinabad, E. Aryan, M. Mobarhan, M. Moohebati, Samaneh Abolbashari, Aida Gholoobi, Amin Houshyar Chechaklou, A. Yaghoubi, M. Meshkat, Z. Meshkat","doi":"10.22037/AMLS.V7.33445","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33445","url":null,"abstract":"Background and Aim: Hepatitis C virus (HCV) infection is one of the leading causes of morbidity and mortality worldwide. It has been hypothesized that a number of bacteria and viruses might be involved in the pathogenesis of cardiovascular disease. The aim of this study was to define the prevalence of HCV in patients with cardiovascular disease in comparison with a control group. \u0000Methods: In this study, 281 individuals including 143 cardiovascular patients and 138 healthy controls were assessed for identification of HCV antibodies. The data collection was done between April 2016 and February 2017. The prevalence of HCV antibodies was determined by the enzyme-linked immunosorbent assay (ELISA) method. \u0000Results: There was no HCV infection in both patients with or without cardiovascular disease. There was a significant direct correlation between cardiovascular diseases and mean level of FPG (Fasting plasma glucose) (p<0.001). Also the Systolic and Diastolic blood pressures were significantly higher in the patients with cardiovascular disease (p<0.001 and p=0.005, respectively). \u0000Conclusion: The results of this study show that no evidence of HCV infection is found among a group of cardiovascular patients in the city of Mashhad. \u0000*Corresponding Author: Zahra Meshkat; Email: meshkatz@mums.ac.ir \u0000Please cite this article as: Shakeri Hoseinabad M, Aryan E, Ghayour Mobarhan M, Moohebati M, Abolbashari S, Gholoobi A, Houshyar Chechaklou A, Yaghoubi A, Meshkat M, Meshkat Z. No Evidence of Hepatitis C Virus Infection in Individuals with Cardiovascular Disease in Mashhad. Arch Med Lab Sci. 2021;7:1-5 (e13). https://doi.org/10.22037/amls.v7.33445","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84359004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Farahnaz Nikdoust, Farzaneh Lashkari Zadeh, S. A. Tabatabaei
Background and Aim: Coronary ectasia is one of the prevalent cardiovascular diseases worldwide. It causes many deaths annually ranged between 9% to 10% which is dependent on related risk factors. The main pathogenesis has not been determined yet; however, it has been shown, that increased lipid profiles and their oxidation in patients can cause endothelial cell dysfunction and thrombosis. Therefore, here we investigated the oxidized phospholipids (OxPLs) role in the pathogenesis of coronary artery disease. Methods: This cross-sectional study was performed in Shariati Hospital, Tehran. Accordingly, patients with coronary artery angiography indications (n=360) were included and classified into one of the following three groups, based on the angiographic results: 1) normal coronary artery (not dysfunction in vessels) or mild CAD (only intimal irregularity or less than 3% narrowing without ectasia); 2) vessel dilation more than 1.5 times compared to the normal part of the vessel or compared to the normal size according to age and gender in one or more coronary vessels; and 3) patients with more than 50% stenosis in one of the coronary arteries. The peripheral blood was collected from patients in EDTA anticoagulants container tubes and the OxPL level was measured using an ELISA kit. Results: The results showed that the amount of OxPL in the third and second groups was higher than in the first group. It was also found that hyperlipidemia, diabetes, hypertension, and smoking were higher in the third and second groups; all mentioned findings were statistically significant (p-value <0.05). Conclusion: According to the findings of this study, it was shown that an increase in OxPL in patients with coronary ectasia can be considered as a risk factor for disease progression; OxPL measurement can be used to identify high-risk individuals. *Corresponding Author: Seyed Abdolhussein Tabatabaei; Email: tabatabaeiseyedah@gmailcom; ORCID iD: 0000-0001-5091-6066 Please cite this article as: Nikdoust F, Lashkari Zadeh F, Tabatabaei SA. Evaluation of OxPL Levels in Patients Undergoing Coronary Angiography: A Cross-Sectional Study. Arch Med Lab Sci. 2021;7:1-6 (e12). https://doi.org/10.22037/amls.v7.34602
{"title":"Evaluation of OxPL Levels in Patients Undergoing Coronary Angiography: A Cross-Sectional Study","authors":"Farahnaz Nikdoust, Farzaneh Lashkari Zadeh, S. A. Tabatabaei","doi":"10.22037/AMLS.V7.34602","DOIUrl":"https://doi.org/10.22037/AMLS.V7.34602","url":null,"abstract":"Background and Aim: Coronary ectasia is one of the prevalent cardiovascular diseases worldwide. It causes many deaths annually ranged between 9% to 10% which is dependent on related risk factors. The main pathogenesis has not been determined yet; however, it has been shown, that increased lipid profiles and their oxidation in patients can cause endothelial cell dysfunction and thrombosis. Therefore, here we investigated the oxidized phospholipids (OxPLs) role in the pathogenesis of coronary artery disease. \u0000Methods: This cross-sectional study was performed in Shariati Hospital, Tehran. Accordingly, patients with coronary artery angiography indications (n=360) were included and classified into one of the following three groups, based on the angiographic results: 1) normal coronary artery (not dysfunction in vessels) or mild CAD (only intimal irregularity or less than 3% narrowing without ectasia); 2) vessel dilation more than 1.5 times compared to the normal part of the vessel or compared to the normal size according to age and gender in one or more coronary vessels; and 3) patients with more than 50% stenosis in one of the coronary arteries. The peripheral blood was collected from patients in EDTA anticoagulants container tubes and the OxPL level was measured using an ELISA kit. \u0000Results: The results showed that the amount of OxPL in the third and second groups was higher than in the first group. It was also found that hyperlipidemia, diabetes, hypertension, and smoking were higher in the third and second groups; all mentioned findings were statistically significant (p-value <0.05). \u0000Conclusion: According to the findings of this study, it was shown that an increase in OxPL in patients with coronary ectasia can be considered as a risk factor for disease progression; OxPL measurement can be used to identify high-risk individuals. \u0000*Corresponding Author: Seyed Abdolhussein Tabatabaei; Email: tabatabaeiseyedah@gmailcom; ORCID iD: 0000-0001-5091-6066 \u0000Please cite this article as: Nikdoust F, Lashkari Zadeh F, Tabatabaei SA. Evaluation of OxPL Levels in Patients Undergoing Coronary Angiography: A Cross-Sectional Study. Arch Med Lab Sci. 2021;7:1-6 (e12). https://doi.org/10.22037/amls.v7.34602","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85923915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ahmadi, H. Maghsoudi, G. Khaniki, R. Hosseini, Parviz Ahangar
Background and Aim: Osteoarthritis (OA) is a chronic inflammatory disease that causes dysfunction of cartilage and joints. Activation of the immune system and the development of inflammatory responses are among the main pathogenesis of the disease. Recently, it has been shown that the expression of Matrix metalloproteinase (MMPs) in patients is increased and leads to the destruction of cartilage in the joints of patients. The introduction of Nanoparticles (NPs) has transformed many fields like medicine, nutrition, and electronics. The usage of nanotechnology in medicine particularly for drug delivery is revealed to have numerous benefits. Therefore, in this study, for the first time, we evaluated the use of Menthol and silver nanoparticles on the expression of MMPs type 1, 3, 9 and 13 in OA models. Methods: BFLS cells were first incubated with LPS to induce inflammation. Then, the expression of MMPs type 1, 2, 3, and 9 was evaluated using RT-PCR, ELISA, and migration and invasion assay. This work, therefore, was aimed to synthesize Menthol-mediated silver nanoparticles and evaluate their anti-inflammatory activity. Menthol mediated silver nanoparticles were synthesized by short-term (1 day) interaction of Menthol (1 mL) with 2mM AgNO3 solution and centrifuged to obtain silver nanoparticles. Further, the menthol mediated AgNPs were evaluated for anti-inflammatory activity by in vitro method. Results: The results showed that the expression of MMPs in LPS-treated cells and silver menthol nanoparticles was decreased compared to LPS-treated cells. In addition, it was shown that menthol silver nanoparticles had the greatest reduction in MMP9 expression and reduced their migration and invasion. Conclusion: It can be said that the use of menthol silver nanoparticles can be used as a supplement along with corticosteroid and anti-inflammatory drugs and even to replace them for the treatment of OA patients, which requires further studies in the future. *Corresponding Author: Hossein Maghsoudi; Email: h.maghsoudi@pnu.ac.ir; ORCID iD: 0000-0002-1524-6420 Please cite this article as: Ahmadi M, Maghsoudi H, Bakhshi Khaniki G, Haj Hosseini R. The effect of Ag-NPs-Menthol on the Expression of Metalloproteinase in the Model-Like Osteoarthritis. Arch Med Lab Sci. 2021;7:1-13 (e11). https://doi.org/10.22037/amls.v7.34925
{"title":"The effect of Ag-NPs-Menthol on the Expression of Metalloproteinase in A Model-Like Osteoarthritis","authors":"M. Ahmadi, H. Maghsoudi, G. Khaniki, R. Hosseini, Parviz Ahangar","doi":"10.22037/AMLS.V7.34925","DOIUrl":"https://doi.org/10.22037/AMLS.V7.34925","url":null,"abstract":"Background and Aim: Osteoarthritis (OA) is a chronic inflammatory disease that causes dysfunction of cartilage and joints. Activation of the immune system and the development of inflammatory responses are among the main pathogenesis of the disease. Recently, it has been shown that the expression of Matrix metalloproteinase (MMPs) in patients is increased and leads to the destruction of cartilage in the joints of patients. The introduction of Nanoparticles (NPs) has transformed many fields like medicine, nutrition, and electronics. The usage of nanotechnology in medicine particularly for drug delivery is revealed to have numerous benefits. \u0000Therefore, in this study, for the first time, we evaluated the use of Menthol and silver nanoparticles on the expression of MMPs type 1, 3, 9 and 13 in OA models. \u0000Methods: BFLS cells were first incubated with LPS to induce inflammation. Then, the expression of MMPs type 1, 2, 3, and 9 was evaluated using RT-PCR, ELISA, and migration and invasion assay. This work, therefore, was aimed to synthesize Menthol-mediated silver nanoparticles and evaluate their anti-inflammatory activity. Menthol mediated silver nanoparticles were synthesized by short-term (1 day) interaction of Menthol (1 mL) with 2mM AgNO3 solution and centrifuged to obtain silver nanoparticles. Further, the menthol mediated AgNPs were evaluated for anti-inflammatory activity by in vitro method. \u0000Results: The results showed that the expression of MMPs in LPS-treated cells and silver menthol nanoparticles was decreased compared to LPS-treated cells. In addition, it was shown that menthol silver nanoparticles had the greatest reduction in MMP9 expression and reduced their migration and invasion. \u0000Conclusion: It can be said that the use of menthol silver nanoparticles can be used as a supplement along with corticosteroid and anti-inflammatory drugs and even to replace them for the treatment of OA patients, which requires further studies in the future. \u0000*Corresponding Author: Hossein Maghsoudi; Email: h.maghsoudi@pnu.ac.ir; ORCID iD: 0000-0002-1524-6420 \u0000Please cite this article as: Ahmadi M, Maghsoudi H, Bakhshi Khaniki G, Haj Hosseini R. The effect of Ag-NPs-Menthol on the Expression of Metalloproteinase in the Model-Like Osteoarthritis. Arch Med Lab Sci. 2021;7:1-13 (e11). https://doi.org/10.22037/amls.v7.34925","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"489 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78406489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background and Aim: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder among women of reproductive age. The hypothalamus-pituitary-ovary axis dysregulates in PCOS, ultimately leading to hyperandrogenisms. Consequently, it is associated with hirsutism, dyslipidemia, obesity, infertility, menstrual disturbance, and insulin resistance. To our knowledge, there is no detailed investigation of these manifestations. That is why, this study aimed to evaluate manifestations of hyperandrogenism, hirsutism in particular. Methods: This study was performed on 39 women with an initial diagnosis of polycystic ovary syndrome. Rotterdam criteria including Oligo- or anovulation, hyperandrogenism clinical and/or biochemical signs, and ultrasound appearance of polycystic ovaries used as diagnostic criteria. Hirsutism scoring was carried out according to Freeman-Gallwey’s definition of hirsutism. The patients were analyzed for other skin problems including male pattern baldness, acanthosis nigricans, and acne as well as demographic features. Results: Hirsutism was observed in the majority of our patients (91%) ranging from mild to severe. Concerning the distribution of hirsutism on various parts of the body, severe hirsutism was observed more on the groin, abdominal area, and chin respectively. Male pattern baldness, acanthosis nigricans, and acne, and dysregulated menstruation were reported in these patients. Conclusion: This investigation found that skin manifestations, especially hirsutism, are highly frequent in patients with PCOS. *Corresponding Author: Hashem Nayeri; Email: hnaieri@gmail.com; nayeri@iaufala.ac.ir Please cite this article as: Masaeli A, Nayeri H, Mirzaei M. A Detailed Cutaneous Manifestations Evaluation in Patients with Polycystic Ovary Syndrome. Arch Med Lab Sci. 2021;7:1-6 (e10). https://doi.org/10.22037/amls.v7.30828
背景与目的:多囊卵巢综合征(PCOS)是育龄妇女最常见的内分泌疾病。下丘脑-垂体-卵巢轴在多囊卵巢综合征中失调，最终导致雄激素过多。因此，它与多毛症、血脂异常、肥胖、不育、月经紊乱和胰岛素抵抗有关。据我们所知，没有详细的调查这些表现。这就是为什么本研究旨在评估雄激素分泌过多的表现，特别是多毛症。方法:本研究对39例初步诊断为多囊卵巢综合征的妇女进行了研究。鹿特丹标准包括少排卵或无排卵、雄激素过多的临床和/或生化征象，以及多囊卵巢超声表现作为诊断标准。根据Freeman-Gallwey对多毛症的定义进行多毛症评分。分析患者的其他皮肤问题，包括男性型秃顶、黑棘皮病、痤疮以及人口统计学特征。结果:在我们的大多数患者(91%)中观察到多毛症，从轻度到重度不等。从多毛在身体各部位的分布情况来看，严重多毛的部位分别是腹股沟、腹部和下巴。男性型秃顶，黑棘皮病，痤疮和月经失调的报道在这些患者。结论:本研究发现多囊卵巢综合征患者的皮肤表现，尤其是多毛症是非常常见的。*通讯作者:Hashem Nayeri;电子邮件:hnaieri@gmail.com;nayeri@iaufala.ac.ir请引用本文:Masaeli A, Nayeri H, Mirzaei M. A多囊卵巢综合征患者的详细皮肤表现评估。中华医学杂志，2011;7:1-6 (e10)。https://doi.org/10.22037/amls.v7.30828
{"title":"A Detailed Cutaneous Manifestations Evaluation in Patients with Polycystic Ovary Syndrome","authors":"Asiyeh Masaeli, H. Nayeri, M. Mirzaei","doi":"10.22037/AMLS.V7.30828","DOIUrl":"https://doi.org/10.22037/AMLS.V7.30828","url":null,"abstract":"Background and Aim: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder among women of reproductive age. The hypothalamus-pituitary-ovary axis dysregulates in PCOS, ultimately leading to hyperandrogenisms. Consequently, it is associated with hirsutism, dyslipidemia, obesity, infertility, menstrual disturbance, and insulin resistance. To our knowledge, there is no detailed investigation of these manifestations. That is why, this study aimed to evaluate manifestations of hyperandrogenism, hirsutism in particular. \u0000Methods: This study was performed on 39 women with an initial diagnosis of polycystic ovary syndrome. Rotterdam criteria including Oligo- or anovulation, hyperandrogenism clinical and/or biochemical signs, and ultrasound appearance of polycystic ovaries used as diagnostic criteria. Hirsutism scoring was carried out according to Freeman-Gallwey’s definition of hirsutism. The patients were analyzed for other skin problems including male pattern baldness, acanthosis nigricans, and acne as well as demographic features. \u0000Results: Hirsutism was observed in the majority of our patients (91%) ranging from mild to severe. Concerning the distribution of hirsutism on various parts of the body, severe hirsutism was observed more on the groin, abdominal area, and chin respectively. Male pattern baldness, acanthosis nigricans, and acne, and dysregulated menstruation were reported in these patients. \u0000Conclusion: This investigation found that skin manifestations, especially hirsutism, are highly frequent in patients with PCOS. \u0000*Corresponding Author: Hashem Nayeri; Email: hnaieri@gmail.com; nayeri@iaufala.ac.ir \u0000Please cite this article as: Masaeli A, Nayeri H, Mirzaei M. A Detailed Cutaneous Manifestations Evaluation in Patients with Polycystic Ovary Syndrome. Arch Med Lab Sci. 2021;7:1-6 (e10). https://doi.org/10.22037/amls.v7.30828","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73683264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Z. Badiei, Sanaz Ahmadi-Ghezaldasht, H. Sima, M. Habibi, M. Khamooshi, A. Azimi, M. Hedayati-Moghaddam
Background and Aim: Patients with blood coagulopathies treated with multiple transfusions have a high risk to acquire some viral infections such as hepatitis C. This research was aimed to identify hepatitis C virus (HCV) infection prevalence, and the viral genotypes among patients with hemophilia and other inherited coagulopathies in Mashhad, Iran. Methods: Medical records of 760 patients with inherited coagulopathies including hemophilia were reviewed in Sarvar Clinic of Mashhad. Plasma samples were subjected to detect antibodies against HCV (anti-HCV) by enzyme-linked immunosorbent assay. HCV RNA and genotypes were determined by a real-time polymerase chain reaction (PCR) method. Results: Totally 128 individuals (16.8%) including patients with hemophilia (n=116) and individuals with other coagulopathies (n=12) were found to be seropositive for anti-HCV. They comprised 122 men and six women with a mean age of 31.6 ± 10.5 years. The PCR results were available for 118 patients, of whom 86 individuals (72.9%) were found to have detectable HCV RNA in their serum. The most frequent genotypes were 1a and 3a (49.1% and 35.8%, respectively). HCV genotypes were not significantly correlated with the patients’ age (p=0.477) as well as with the serum levels of alanine aminotransferase (p=0.655) and aspartate aminotransferase (p=0.332). Conclusion: The patients with blood coagulation disorders had a greater prevalence of HCV infection in comparison with the general population in our region. The most common subgenotypes of HCV were 1a, and 3a, respectively. These results could assist professionals to choose more efficient approaches for the management of their patients. *Corresponding Author: Mohammad Reza Hedayati-Moghaddam; Email: drhedayati@acecr.ac.ir Please cite this article as: Badiei Z, Ahmadi-Ghezeldasht S, Sima HR, Habibi M, Khamooshi M, Azimi A, Hedayati-Moghaddam MR. The Prevalence of Hepatitis C Virus (HCV) Infection and Genotypes in Patients with Hemophilia and Other Blood Coagulopathies in Mashhad, Iran. Arch Med Lab Sci. 2021;7:1-7 (e9). https://doi.org/10.22037/amls.v7.33968
背景与目的:在伊朗马什哈德，血友病和其他遗传性凝血疾病患者中，多次输血治疗的凝血功能障碍患者获得某些病毒感染(如丙型肝炎)的风险很高。本研究旨在确定丙型肝炎病毒(HCV)感染的流行情况和病毒基因型。方法:对马什哈德Sarvar诊所760例包括血友病在内的遗传性凝血病患者的病历进行回顾性分析。血浆样品采用酶联免疫吸附法检测抗HCV抗体。实时聚合酶链反应(PCR)检测HCV RNA和基因型。结果:共有128人(16.8%)，包括血友病患者(n=116)和其他凝血疾病患者(n=12)，发现抗- hcv血清阳性。男性122人，女性6人，平均年龄31.6±10.5岁。PCR结果可用于118例患者，其中86例(72.9%)血清中检测到HCV RNA。最常见的基因型为1a和3a(分别为49.1%和35.8%)。HCV基因型与患者年龄(p=0.477)、血清丙氨酸转氨酶(p=0.655)、天冬氨酸转氨酶(p=0.332)无显著相关。结论:本地区凝血功能障碍患者的HCV感染率高于普通人群。HCV最常见的亚基因型分别为1a和3a。这些结果可以帮助专业人员选择更有效的方法来管理他们的病人。*通讯作者:Mohammad Reza Hedayati-Moghaddam;Badiei Z, Ahmadi-Ghezeldasht S, Sima HR, Habibi M, Khamooshi M, Azimi A, Hedayati-Moghaddam MR.伊朗马什哈德血友病和其他凝血疾病患者丙型肝炎病毒(HCV)感染的患病率和基因型。中华医学杂志，2021;7:1-7(9)。https://doi.org/10.22037/amls.v7.33968
{"title":"The Prevalence of Hepatitis C Virus (HCV) Infection and Genotypes in Patients with Hemophilia and Other Blood Coagulopathies in Mashhad, Iran","authors":"Z. Badiei, Sanaz Ahmadi-Ghezaldasht, H. Sima, M. Habibi, M. Khamooshi, A. Azimi, M. Hedayati-Moghaddam","doi":"10.22037/AMLS.V7.33968","DOIUrl":"https://doi.org/10.22037/AMLS.V7.33968","url":null,"abstract":"Background and Aim: Patients with blood coagulopathies treated with multiple transfusions have a high risk to acquire some viral infections such as hepatitis C. This research was aimed to identify hepatitis C virus (HCV) infection prevalence, and the viral genotypes among patients with hemophilia and other inherited coagulopathies in Mashhad, Iran. \u0000Methods: Medical records of 760 patients with inherited coagulopathies including hemophilia were reviewed in Sarvar Clinic of Mashhad. Plasma samples were subjected to detect antibodies against HCV (anti-HCV) by enzyme-linked immunosorbent assay. HCV RNA and genotypes were determined by a real-time polymerase chain reaction (PCR) method. \u0000Results: Totally 128 individuals (16.8%) including patients with hemophilia (n=116) and individuals with other coagulopathies (n=12) were found to be seropositive for anti-HCV. They comprised 122 men and six women with a mean age of 31.6 ± 10.5 years. The PCR results were available for 118 patients, of whom 86 individuals (72.9%) were found to have detectable HCV RNA in their serum. The most frequent genotypes were 1a and 3a (49.1% and 35.8%, respectively). HCV genotypes were not significantly correlated with the patients’ age (p=0.477) as well as with the serum levels of alanine aminotransferase (p=0.655) and aspartate aminotransferase (p=0.332). \u0000Conclusion: The patients with blood coagulation disorders had a greater prevalence of HCV infection in comparison with the general population in our region. The most common subgenotypes of HCV were 1a, and 3a, respectively. These results could assist professionals to choose more efficient approaches for the management of their patients. \u0000*Corresponding Author: Mohammad Reza Hedayati-Moghaddam; Email: drhedayati@acecr.ac.ir \u0000Please cite this article as: Badiei Z, Ahmadi-Ghezeldasht S, Sima HR, Habibi M, Khamooshi M, Azimi A, Hedayati-Moghaddam MR. The Prevalence of Hepatitis C Virus (HCV) Infection and Genotypes in Patients with Hemophilia and Other Blood Coagulopathies in Mashhad, Iran. Arch Med Lab Sci. 2021;7:1-7 (e9). https://doi.org/10.22037/amls.v7.33968","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"164 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86958546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Eslamnik, Mana Moghaddasi, Ali Malmir, Bahar Sadeghi-Goughari, M. Javan, M. Ebrahimi
Today, young women infertility through chemotherapy has become a global challenge. Chemotherapy destructs the malignant cells by reactive oxygen species (ROS) production and inflammatory factors secretion; these factors can also destruct the ovarian and uterine cells. Infertility usually happens as a result of ovarian and uterine cells apoptosis, as well as dysfunction in these organs. Signaling pathways activated by chemotherapy lead to increased activation of follicles and depletion of the follicular pool. In addition, excessive secretion of sex hormones leads to follicles activation and infertility in women. Mesenchymal stem cells (MSC) use have been reported to be a great help in restoring the function of ovarian and uterine cells. On the other hand, they can regulate sex hormone secretion. Finally, the use of MSCs as a suitable treatment strategy can help restore the function of reproductive cells and treat infertility. *Corresponding Author: Maryam Ebrahimi; ORCID iD: 0000 0001 9685 7882; Email: drmkhoddami@yahoo.com Please cite this article as: Eslamnik P, Moghaddasi M, Malmir A, Sadeghi-Goughari B, Javan MR, Ebrahimi M. The Effect of Chemotherapy in Gestational Trophoblastic Disease on Infertility: A Review on Pathophysiologic Mechanism. Arch Med Lab Sci. 2021;7:1-8 (e8). https://doi.org/10.22037/amls.v7.34369
如今，年轻女性通过化疗不孕不育已成为一个全球性的挑战。化疗通过活性氧(ROS)的产生和炎性因子的分泌来破坏恶性细胞;这些因素也会破坏卵巢和子宫细胞。不孕症通常是由于卵巢和子宫细胞凋亡以及这些器官功能障碍造成的。化疗激活的信号通路导致卵泡激活增加和卵泡池耗竭。此外，性激素分泌过多会导致卵泡激活和女性不孕。间充质干细胞(MSC)在恢复卵巢和子宫细胞功能方面有很大的帮助。另一方面，它们可以调节性激素的分泌。最后，使用间充质干细胞作为一种合适的治疗策略，可以帮助恢复生殖细胞的功能，治疗不孕症。*通讯作者:Maryam Ebrahimi;ORCID iD: 0000 0001 9685 7882;Eslamnik P, Moghaddasi M, Malmir A, Sadeghi-Goughari B, Javan MR, Ebrahimi M.妊娠滋养细胞疾病化疗对不孕症的影响:病理生理机制综述。中华医学杂志，2021;7:1-8 (e8)。https://doi.org/10.22037/amls.v7.34369
{"title":"The Effect of Chemotherapy in Gestational Trophoblastic Disease on Infertility: A Review on Pathophysiologic Mechanism","authors":"P. Eslamnik, Mana Moghaddasi, Ali Malmir, Bahar Sadeghi-Goughari, M. Javan, M. Ebrahimi","doi":"10.22037/AMLS.V7.34369","DOIUrl":"https://doi.org/10.22037/AMLS.V7.34369","url":null,"abstract":"Today, young women infertility through chemotherapy has become a global challenge. Chemotherapy destructs the malignant cells by reactive oxygen species (ROS) production and inflammatory factors secretion; these factors can also destruct the ovarian and uterine cells. \u0000Infertility usually happens as a result of ovarian and uterine cells apoptosis, as well as dysfunction in these organs. Signaling pathways activated by chemotherapy lead to increased activation of follicles and depletion of the follicular pool. In addition, excessive secretion of sex hormones leads to follicles activation and infertility in women. \u0000Mesenchymal stem cells (MSC) use have been reported to be a great help in restoring the function of ovarian and uterine cells. On the other hand, they can regulate sex hormone secretion. Finally, the use of MSCs as a suitable treatment strategy can help restore the function of reproductive cells and treat infertility. \u0000*Corresponding Author: Maryam Ebrahimi; ORCID iD: 0000 0001 9685 7882; Email: drmkhoddami@yahoo.com \u0000Please cite this article as: Eslamnik P, Moghaddasi M, Malmir A, Sadeghi-Goughari B, Javan MR, Ebrahimi M. The Effect of Chemotherapy in Gestational Trophoblastic Disease on Infertility: A Review on Pathophysiologic Mechanism. Arch Med Lab Sci. 2021;7:1-8 (e8). https://doi.org/10.22037/amls.v7.34369","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80098849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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