Antimicrobial Capacity, Antioxidant and Free Radical Scavenging Activity of Extract from the Leaf of Erigeron floribundus

O. Bola, F. B. Ajimojuowo, O. T. Oyekale
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Abstract

Fresh leaves of Erigeron floribundus were obtained, and its ethanolic extract was assessed for antimicrobial, antioxidant-free radical scavenging activity as well as phytochemical constituents. Hemolytic activities were also screened to know whether the extract will lyse the blood agar. Six pathogenic bacteria; the gram-positive, Bacillus cereus, Clostridium species, Staphylococcus aureus, and the gram-negative: Klebsiella pneumonia, Pseudomonas aeruginosa, and Escherichia coli, were screened for susceptibilities to the antimicrobial activity of the leaf extract. Five fungi, Yeast, Penicillium sp, Aspergillus niger, Aspergillus flavus, and Aspergillus fumigatus, were also screened for antifungal properties of the extract. The result indicated that the plant extract showed antimicrobial action but to a varying degree. The extract was screened for its antioxidant property and free radical scavenging capacity. The result indicated that it has high activity in reducing iron chloride solution and high activity in (DPPH) radicals scavenging test. The phytochemical screening of the extracts showed some bioactive ingredient of pharmacological importance like Tannin, Cardiaglycosides, and Saponin. Hemolytic assay of the extract showed that it could lyse blood cells.
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鸢尾叶提取物的抗微生物能力、抗氧化能力及自由基清除能力
以鲜雏菊(Erigeron floribundus)叶片为原料,对其乙醇提取物的抗微生物活性、抗氧化自由基清除活性及植物化学成分进行了评价。还对溶血活性进行了筛选,以了解提取物是否会溶解血琼脂。六种致病菌;对革兰氏阳性菌蜡样芽孢杆菌、梭状芽孢杆菌、金黄色葡萄球菌和革兰氏阴性菌肺炎克雷伯菌、铜绿假单胞菌和大肠杆菌进行了抗菌活性筛选。对酵母、青霉、黑曲霉、黄曲霉和烟曲霉等5种真菌进行了抑菌性筛选。结果表明,该植物提取物具有不同程度的抗菌作用。对其抗氧化性能和自由基清除能力进行了筛选。结果表明,它对氯化铁溶液具有较高的还原活性,对DPPH自由基具有较高的清除活性。通过植物化学筛选,发现了具有重要药理作用的单宁、心糖苷、皂苷等生物活性成分。溶血实验表明,该提取物具有溶解血细胞的作用。
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