Shinjiro Kobayashi, M. Nomura, Tatsuo Takahashi, Miho Suzuki, R. Nagai, N. Hagino
{"title":"Nε-(Carboxymethyl)Lysine-Induced Choroidal Angiogenic Potential Facilitates Retinal Neovascularization in Advanced-Diabetic Rat In Vitro","authors":"Shinjiro Kobayashi, M. Nomura, Tatsuo Takahashi, Miho Suzuki, R. Nagai, N. Hagino","doi":"10.2174/1874143600802010079","DOIUrl":null,"url":null,"abstract":"N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic and age-matched normal rats. When retinal explant was co-cultured with early-diabetic choroidal explant, the number of retinal microvessels was significantly decreased. When retinal ex- plant was co-cultured with advanced-diabetic choroidal explant, the number of retinal microvessels was significantly in- creased. Anti-CML antibody blocked the retinal changes caused by co-culture with both early-diabetic and advanced- diabetic choroidal explant. Antibodies against TNF and VEGF reduced the number of retinal microvessels in the co- culture with advanced-diabetic choroidal explant. These results indicate that the CML-induced choroidal activity is asso- ciated with the angiogenic actions of TNF and VEGF on retinal capillaries in advanced diabetes. During the course of diabetic retinopathy, different actions on retinal neovascularization may operate.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"93 1","pages":"79-86"},"PeriodicalIF":0.0000,"publicationDate":"2008-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Pharmacology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874143600802010079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
N -(Carboxymethyl)lysine (CML) over-stimulates choroidal neovascularization in vitro in streptozotocin (STZ)-diabetic rat. In this study, we investigated the effects of CML-induced choroidal angiogenic potential on retinal neovascularization during the course of STZ-induced diabetes in rats. Retinal and choroidal explants were isolated from the same eyeball of early-diabetic, advanced-diabetic and age-matched normal rats. When retinal explant was co-cultured with early-diabetic choroidal explant, the number of retinal microvessels was significantly decreased. When retinal ex- plant was co-cultured with advanced-diabetic choroidal explant, the number of retinal microvessels was significantly in- creased. Anti-CML antibody blocked the retinal changes caused by co-culture with both early-diabetic and advanced- diabetic choroidal explant. Antibodies against TNF and VEGF reduced the number of retinal microvessels in the co- culture with advanced-diabetic choroidal explant. These results indicate that the CML-induced choroidal activity is asso- ciated with the angiogenic actions of TNF and VEGF on retinal capillaries in advanced diabetes. During the course of diabetic retinopathy, different actions on retinal neovascularization may operate.