The potential effect of temulawak (Curcuma xanthorrizha Roxb) and garlic (Allium sativum L.) as a radioprotective agent against 6 Gy total body irradiation in rats

IF 1.4 4区医学 Q4 ENVIRONMENTAL SCIENCES Radioprotection Pub Date : 2023-07-31 DOI:10.1051/radiopro/2023024

T. Kisnanto, D. Tetriana, D. Yusuf, Y. Lusiyanti, HNE. Surniyantoro, IK Hasan Basri

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Abstract

The main impact of gamma radiation on a biological system is the formation of Reactive Oxygen Species (ROS) and free radicals. The study aimed to explore the potential effect of temulawak (Curcuma xanthorrizha Roxb) and garlic (Allium sativum L) against 6 Gy total body irradiation (TBI) in rats by observing malondialdehide (MDA), glutathione (GSH) and comet assay. Twenty male rats were divided into five groups: control, 6 Gy, temulawak extract+6 Gy, garlic extract+6 Gy, and n-acetyl cysteine (NAC)+6 Gy. MDA and GSH were measured on liver and spleen tissue homogenates, while comet assay was on lymphocyte cells. Gamma irradiation at 6 Gy significantly increased the MDA level and comet assay compared to the control group, while the GSH level decreased (p < 0.05). Temulawak extract significantly reduced MDA levels and comet assay compared to the 6 Gy group, while GSH levels in the liver increased. Garlic extract significantly drops comet assay while increasing GSH levels in the liver. NAC decreases MDA levels in the liver and comet assay while increasing GSH levels in the spleen. It could be concluded that temulawak extract has a better radioprotective agent than garlic extract and is almost identical to NAC as a standard antioxidant.

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莪术(Curcuma xanthorrizha Roxb)和大蒜(Allium sativum L.)作为大鼠抗6 Gy全身辐射防护剂的潜在作用

伽马辐射对生物系统的主要影响是活性氧(ROS)和自由基的形成。本研究旨在通过丙二醛(MDA)、谷胱甘肽(GSH)和彗星试验，探讨黄姜黄(Curcuma xanthorrizha Roxb)和大蒜(Allium sativum L)对大鼠6 Gy全身辐照(TBI)的潜在影响。雄性大鼠20只，随机分为5组:对照组、6 Gy组、地木参提取物+6 Gy组、大蒜提取物+6 Gy组、n-乙酰半胱氨酸(NAC)+6 Gy组。肝、脾组织匀浆检测丙二醛和谷胱甘肽，淋巴细胞检测彗星试验。与对照组相比，6 Gy γ辐照显著提高了MDA水平和彗星测定值，降低了GSH水平(p < 0.05)。与6 Gy组相比，Temulawak提取物显著降低MDA水平和彗星测定，同时肝脏GSH水平升高。大蒜提取物显著降低彗星测定，同时增加肝脏中谷胱甘肽水平。NAC降低肝脏和彗星试验中的丙二醛水平，同时增加脾脏中的谷胱甘肽水平。综上所述，铁木犀草提取物具有比大蒜提取物更好的防辐射作用，其抗辐射作用与NAC基本一致。

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来源期刊

Radioprotection ENVIRONMENTAL SCIENCES-PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

CiteScore

3.30

自引率

54.50%

发文量

审稿时长

>12 weeks

期刊介绍： Radioprotection publishes articles on all aspects of radiological protection, including non-ionising as well as ionising radiations. Fields of interest range from research, development and theory to operational matters, education and training. The very wide spectrum of its topics includes (theoretical and practical aspects): dosimetry, instrument development, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.