교배형 분자마커를 이용한 신품종 밀리타리스 동충하초 ‘도원홍초 2호’의 품종 특성

이병주, 이미애, 김용균, 이순계, 최영상, 이병의
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Abstract

The mushroom species Cordyceps militaris has been studied and cultivated as a medicinal mushroom due to its multiple valuable biological and pharmaceutical activities. For breeding new strains of C. militaris, multiplex PCR assays were performed using primers specific for its mating type genes, MAT1-1 and MAT1-2. Mating types and mating status were confirmed, as evidenced by DNA bands at 233-bp and 191-bp for MAT1-1 and MAT1-2 respectively. The novel 'Dowonhongcho 2ho’ was developed through mating; they were found to possess high-quality fruiting bodies when grown in artificial media. The stromata of the new strain were club-shaped, with a bright orange-red color, and measured 7.1 cm in length. They had an average cordycepin content of 0.33%. Compared to 'Dowonhongcho,' the new strain had a 7% higher yield, as well as firm fruiting bodies. The optimum temperature for mycelial growth was 20~25°C, and the optimum temperature for stroma development was 18~22 °C. The fruiting bodies developed after 49.1 days from inoculation. The use of mating type molecular markers improved the breeding efficiency of the new strain 'Dowonhongcho 2ho.’ Thus, they may be valuable for artificial cultivation and industrial-scale production of C. militaris with excellent characteristics.
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利用交配型分子标记的新品种冬虫夏草“桃源红草2号”的品种特性
由于蛹虫草具有多种有价值的生物活性和药理活性,已被作为药用蘑菇进行了研究和栽培。为选育新菌株,采用其交配型基因MAT1-1和MAT1-2特异性引物进行多重PCR检测。MAT1-1和MAT1-2的DNA条带分别为233-bp和191-bp,证实了交配类型和交配状态。小说《下洪草2》是通过交配发展起来的;当在人工培养基中生长时,发现它们具有高质量的子实体。新菌株的基质呈棒状,呈明亮的橙红色,长7.1 cm。虫草素平均含量为0.33%。与“Dowonhongcho”相比,新品系的产量提高了7%,而且子实体结实。菌丝生长的最适温度为20~25℃,基质发育的最适温度为18~22℃。接种后49.1 d子实体发育。配偶型分子标记的使用提高了新品系“稻红草2ho”的育种效率。因此,它们具有优良的特性,可用于人工栽培和工业规模生产。
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