Assessment of Temperature and Metallic Stresses on a Psychrotolerant Bacterium Pseudomonas veronii ASP36 Isolated from Himalayan Region in Uttarakhand State, India

A. S. Panwar, G. K. Joshi
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Abstract

Bacteria are one of the most versatile living creatures having ability to sustain life processes under various suboptimal conditions. Present work illustrates the impact of temperature and metallic stresses on a psychrotolerant bacterium isolated from Himalayan region in India. The bacterium was morphologically and biochemically characterized and identified as Pseudomonas veronii ASP36 based on the 16S rDNA sequencing followed by BLAST similarity search. Growth at low temperature incited the bacterium to produce more exopolysaccharides. Preincubation of the bacterium at near zero temperature enhanced its freeze tolerance. ZnSO4 induced genomic changes in the test isolate were revealed through RAPD.
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印度北阿坎德邦喜玛拉雅地区耐寒细菌维罗尼假单胞菌ASP36的温度和金属胁迫评价
细菌是最多才多艺的生物之一,有能力在各种次优条件下维持生命过程。目前的工作说明了温度和金属应力对从印度喜马拉雅地区分离的耐寒细菌的影响。通过16S rDNA测序和BLAST相似性搜索,鉴定该细菌为veronii假单胞菌ASP36。低温下的生长刺激细菌产生更多的胞外多糖。在接近零度的温度下进行预孵育,增强了细菌的抗冻能力。RAPD分析了ZnSO4诱导的基因组变化。
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