Antibacterial Effects of Various Concentrations of Natural Ingredients Snail Mucus (Achatina fulica) Against Inhibition Zones of Fusobacterium nucleatum Causes Periodontitis In Vitro

Swastini Igaap, Ni Nengah Sumerti
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Abstract

Periodontitis is a microorganism that invades the subgingival area, thereby triggering an inflammatory response of the periodontal tissue. Fusobacterium nucleatum is one of the most abundant gram-negative bacilli colonizing the subgingival plaque and closely associated with periodontal disease. The aim of study was to calculate the inhibition of snail mucus against Fusobacterium nucleatum bacteria with concentrations of 12.5%, 25%, 50%, and 100%. This study used a laboratory experimental test method and was conducted at the Oral Biology Laboratory, Faculty of Dentistry, Airlangga University. The steps were performed are: plant germ stock with sterile osse on BHI broth media then incubate for 48 hours, observe the turbidity of the germs then standardize with mc farland standard 0.5, plant germs on Hilton Muller agar media with the spreading technique, treat the test sample on sterile paper disk as much as 0, 01 ml with a sterile micropipette, then paste it on the surface of the media so that it is then incubated for 48 hours. Then, observe and measure the diameter of the clear zone. The results show that the average inhibition in the control group is 25.65 mm, and the snail mucus group is 12.5%, 0 mm, 25%, 12.40 mm, 50%, 16.70 mm and 100% 19.60 mm. There is a significant difference in the diameter of the inhibitory power between the treatment groups. The conclusion of the antibacterial effect of natural ingredients of snail mucus from the 25% treatment showed strong criteria, and the greatest inhibitory power was the concentration of 100%.
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不同浓度天然成分蜗牛黏液对牙周炎梭杆菌抑菌带的体外抑菌作用
牙周炎是一种微生物侵入牙龈下区域,从而引发牙周组织的炎症反应。核梭杆菌是龈下菌斑中数量最多的革兰氏阴性杆菌之一,与牙周病密切相关。研究的目的是计算蜗牛黏液在12.5%、25%、50%和100%浓度下对核梭杆菌的抑制作用。本研究采用实验室实验测试方法,在艾尔朗加大学牙科学院口腔生物学实验室进行。步骤为:用无菌骨料的植物胚芽原液在BHI肉汤培养基上孵育48小时,观察菌种的浊度,用mcfarland标准0.5进行标准化,用扩散技术将植物胚芽置于Hilton Muller琼脂培养基上,用无菌微移液管在无菌纸盘上处理至0.01 ml,然后粘贴在培养基表面孵育48小时。然后,观察并测量清晰区的直径。结果表明,对照组的平均抑制率为25.65 mm,蜗牛黏液组的平均抑制率为12.5%、0 mm、25%、12.40 mm、50%、16.70 mm和100% 19.60 mm。两组间抑制力直径差异有统计学意义。钉螺黏液天然成分25%处理的抑菌效果指标较强,抑菌力最大的浓度为100%。
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