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Identification of Candida Isolates from Cancer Patients Using Multiplex PCR 多重PCR法鉴定癌症患者假丝酵母分离株
S. Ganguli, W. Wijendra, P. Dasanayaka, R. Ramesh, A. Kaushalya, S. Gunasekara
Aim of this study was to identify the Candida isolates from Cancer Hospital Maharagama, Sri Lanka by using Multiplex PCR (polymerase chain reaction) method. In this study, Multiplex PCR was used to identify Candida isolates from cancer patients at Apeksha Hospital, Sri Lanka. In multiplex PCR, 18 specific primers (nine primer pairs) were separated into three groups for multiplex PCR (PS I, PS II and PS III). Different sized PCR products corresponding to each Candida species were produced by PCR using the primer mixes. Among 52 Candida isolates, 49 were identified using the multiplex PCR procedure. Candida tropicalis was found to be the most prevalent species (38%), followed by Candida parapsilosis (31%), Candida albicans (13%), Candida glabrata (8%) and Candida krusei (4%). The study concludes that multiplex PCR is a better approach for the identification of Candida species and recommends the use of it for clinical and diagnostic purposes and for research purposes.
本研究的目的是采用多重PCR(聚合酶链反应)方法对斯里兰卡Maharagama肿瘤医院的念珠菌分离株进行鉴定。在这项研究中,多重PCR用于鉴定斯里兰卡Apeksha医院癌症患者的念珠菌分离株。在多重PCR中,将18对特异性引物(9对引物)分成3组(PS I、PS II和PS III)进行多重PCR,利用这些引物混合物进行PCR,得到不同念珠菌种类对应的不同大小的PCR产物。52株念珠菌分离株中,49株经多重PCR鉴定。热带念珠菌最多(38%),其次为假丝酵母菌(31%)、白色念珠菌(13%)、光秃念珠菌(8%)和克鲁氏念珠菌(4%)。该研究得出结论,多重PCR是鉴定念珠菌种类的较好方法,并建议将其用于临床和诊断目的以及研究目的。
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引用次数: 0
Challenges and Strategies on Blood Banking and Transfusion Practices During the COVID-19 Pandemic: Ensuring Safe Use of Blood and Blood Products 2019冠状病毒病大流行期间血库和输血做法的挑战和战略:确保安全使用血液和血液制品
Glenlyd Helmut A. Romanillos, Brian M. Denney, Emmalyn B. Cutamora
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引用次数: 0
Management of Respiratory Failure in Peripartum Cardiomyopathy Patient: Case Report 围生期心肌病患者呼吸衰竭的处理:1例报告
Dewi Yuliana Fithri, Budiana Rismawan
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引用次数: 0
Alternative Antibiotics Therapy for Drug-resistant Gonorrhea: A Literature Review 耐药淋病的替代抗生素治疗:文献综述
Dian Andriani Ratna Dewi, Nabila Arkania, Ni Made Wiliantari, Farrasila Nadhira, Clara Virginia Allun, Arohid Allatib, Cut Annisa Salsabila, Kelvin Dewantara, Angki Perdiyana
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引用次数: 0
Evaluating the Strength of Evidence on the Effectiveness of Accommodative Support Lens on Computer Vision Syndrome 评价可调节支撑晶状体治疗计算机视觉综合征有效性的证据强度
H. Egharevba
Computer vision syndrome is a disease that has emerged with the advent of the computer and digital age. It is most associated with long-hour users of digital screen devices. Its symptoms are ocular and extraocular and may include blur or double vision, and other visual discomforts such as redness, irritation, dry eye, watery eye, headache, neck and shoulder pains, back aches and tendonitis. Several studies have reported a prevalence rate of between 10-90%. Depending on a number of factors, different treatment approaches have been adopted by physicians for primary and secondary care. These approaches include optimization of ergonomics, progressive addition of lenses, use of nutritional supplements, and artificial tears. The validity of the efficacy of these approaches has continued to generate debates among public health stakeholders due to limited evidence. This article discusses the strength of evidence of the use of accommodative support lenses in alleviating the symptoms of computer vision syndrome, with a special focus on the work of Seguí-Crespo et al., 2022.
计算机视觉综合症是随着计算机和数字时代的到来而出现的一种疾病。它与长时间使用数字屏幕设备的用户最为相关。它的症状是眼和眼外,可能包括模糊或重影,以及其他视觉不适,如发红、刺激、干眼、流泪、头痛、颈肩痛、背部疼痛和肌腱炎。几项研究报告的患病率在10-90%之间。根据许多因素,医生对初级和二级护理采用了不同的治疗方法。这些方法包括优化人体工程学、逐步添加镜片、使用营养补充剂和人工泪液。由于证据有限,这些方法有效性的有效性继续在公共卫生利益攸关方中引起争论。本文讨论了使用适应性支持镜片缓解计算机视觉综合征症状的证据强度,特别关注Seguí-Crespo等人,2022年的工作。
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引用次数: 0
Systematic Review in Population-Based General Understanding Surveys of Stroke Perception, Potential Risks, Treatments, and Perspectives for a Correlative Study on Public Awareness Between Different Age Groups and their Lifestyles 以人群为基础的卒中感知、潜在风险、治疗的总体认识调查的系统回顾,以及不同年龄组和生活方式之间公众认知的相关研究的观点
Nathakorn Pattanakul, Pratchayapong Yasri
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引用次数: 0
A Literature Review on Regulations Enforced to Reduce Adolescents’ Use of E-Cigarette in Thailand 泰国减少青少年电子烟使用法规的文献综述
Phudit Pratchyanupat, Abhasnee Sobhonslidsuk
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引用次数: 0
The Impact of Postpartum Mothers' Stress and Motivation on Breastfeeding Practice 产后母亲压力与动机对母乳喂养行为的影响
Maya Astuti, Fuadah Ashri Nurfurqoni, Enung Harni S.
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引用次数: 0
Effect of Repetitive Transcranial Magnetic Stimulation on Change in Delta Theta Alfa Beta Ratio (DTABR) and Motor Function in Ischemic Stroke Patients 反复经颅磁刺激对缺血性脑卒中患者DTABR及运动功能变化的影响
Willy Candra
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引用次数: 0
A Qualitative Study on the Effects of Health Literacy in Population with Hypertension at Blossom Health Care Center, Kabul City, Afghanistan 阿富汗喀布尔市Blossom保健中心高血压人群健康素养影响的定性研究
Ahmad Khan, Melanie M. Tidman, Hasibullah Najib, Ihsanullah Darmal
Hypertension is a chronic disease common in developing countries like Afghanistan, and improving patient awareness of the risk factors and the significance of regular treatment decreases the burden of the disease. In Afghanistan, a higher level of health literacy can help patients with hypertension to know the risk factors, the role of lifestyle management, and the significance of regular treatment for hypertension. Also, a higher level of health literacy can provide venues for the patient to comprehend the provided information and actively participate in the care. Alternatively, patients with lower health literacy might need to appropriately understand the information related to diet, treatment, and provider communication during the management of hypertension. A lack of understanding of hypertension-related treatment suggestions might lead to poor compliance with hypertension medication. This qualitative study aimed to assess the health literacy level among patients seeking care for hypertension at Blossom Health Care Center in Kabul, Afghanistan. A total of 198 patients who had hypertension participated in the study, and patients were interviewed using a Likert scale for responses generated by a focus group of 10 Afghan doctors practicing in Kabul, Afghanistan. We found that 68.7% of patients with hypertension scored Strongly Disagree to having adequate hypertension knowledge. Moreover, around 37.3% of participants indicated Always visiting a doctor for this condition, and 38.4 indicated Always taking their medication. Studies have shown that lower health literacy in patients with hypertension might negatively impact their health outcomes. Therefore, increasing awareness and knowledge of hypertension related-treatment among people with hypertension might improve their overall health and well-being.
高血压是阿富汗等发展中国家常见的慢性疾病,提高患者对危险因素的认识和定期治疗的重要性可以减轻疾病的负担。在阿富汗,提高卫生知识水平可以帮助高血压患者了解危险因素、生活方式管理的作用以及定期治疗高血压的重要性。此外,较高水平的健康素养可以为患者提供理解所提供信息并积极参与护理的场所。另外,健康素养较低的患者可能需要适当了解高血压管理过程中与饮食、治疗和提供者沟通相关的信息。缺乏对高血压相关治疗建议的理解可能导致高血压药物依从性差。本定性研究旨在评估在阿富汗喀布尔Blossom医疗保健中心寻求高血压护理的患者的健康素养水平。共有198名高血压患者参与了这项研究,患者接受了李克特量表的采访,该量表由10名在阿富汗喀布尔执业的阿富汗医生组成的焦点小组生成。我们发现68.7%的高血压患者强烈不同意有足够的高血压知识。此外,约37.3%的参与者表示总是去看医生,38.4%的参与者表示总是服用药物。研究表明,高血压患者较低的健康素养可能会对他们的健康结果产生负面影响。因此,提高高血压患者对高血压相关治疗的认识和知识可能会改善他们的整体健康和福祉。
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引用次数: 0
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World Academy of Science, Engineering and Technology, International Journal of Medical and Health Sciences
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