M. Masri, Cut Muthiadin, M. Masita, T. Cahyanto, L. Lianah, R. Rusny, S. Tridesianti
{"title":"Black Cumin (Nigella sativa) Against Mycobacterium tuberculosis Strain H37RV And MDR-TB","authors":"M. Masri, Cut Muthiadin, M. Masita, T. Cahyanto, L. Lianah, R. Rusny, S. Tridesianti","doi":"10.22373/ekw.v7i1.9335","DOIUrl":null,"url":null,"abstract":"Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of N. sativa seed extract in inhibiting the growth of M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan. Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%, tetapi resisten terhadap ekstrak N. sativa konsentrasi 1 dan 3%.","PeriodicalId":11527,"journal":{"name":"Elkawnie","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Elkawnie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22373/ekw.v7i1.9335","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Abstract: Tuberculosis (TB) is a contagious infectious disease caused by Mycobacterium tuberculosis. 10 million people suffer from TB Every year. Although TB is a preventable and treatable disease, 1.5 million people die every year due to TB. Alternative treatments continue to be pursued, and treatment with the latest TB drugs that are continuously being encouraged. Black cumin (Nigella sativa) seed contains essential oils with active compounds such as thymohydroquinone, Oleoresins, flavonoids, alkaloids, saponins, tannins, and terpenoids that act as antibacterial drugs. This study aims to determine the sensitivity of N. sativa seed extract in inhibiting the growth of M. tuberculosis strain H37RV and MDR-TB (Multidrug Resistance-TB). This research using Microscopic-Observation and Drug-Susceptibility Assay (MODS) method. Extraction of N. sativa was carried out by the maceration method using 70% methanol as a solvent. The results showed that the M. tuberculosis strain H37RV and MDR-TB were sensitive to N. sativa extract at concentrations of 5 and 10% but resistant to N. sativa extract at concentrations of 1 and 3%.Abstrak: Tuberkulosis (TB) adalah penyakit menular yang disebabkan oleh Bakteri Mycobacterium tuberculosis. Penyakit ini menimbulkan dampak kematian yang cukup mengkhawatirkan. Penyakit tersebut dapat dicegah dan diobati. Salah satu sumber pengobatannya menggunakan biji jintan hitam (Nigella sativa) yang mengandung minyak atsiri dengan senyawa aktif seperti timohidrokuinon, oleoresin, flavonoid, alkaloid, saponin, tanin, dan terpenoid yang berfungsi sebagai obat antibakteri. Penelitian ini bertujuan untuk mengetahui sensitivitas ekstrak biji N. sativa dalam menghambat pertumbuhan M. tuberculosis strain H37RV and MDR-TB (Multidrug-Resistance-TB). Penelitian ini menggunakan metode Microscopic-Observation and Drug-Susceptibility Assay (MODS). Ekstraksi N. sativa dilakukan dengan metode maserasi menggunakan pelarut metanol 70%. Hasil yang diperoleh menunjukkan bahwa bakteri M. tuberculosis strain H37RV dan TB-MDR, kedua strain tsb sensitif terhadap ekstrak N. sativa konsentrasi 5 dan 10%, tetapi resisten terhadap ekstrak N. sativa konsentrasi 1 dan 3%.
摘要:结核病(TB)是由结核分枝杆菌引起的传染性传染病。每年有1000万人患结核病。虽然结核病是一种可预防和可治疗的疾病，但每年仍有150万人死于结核病。继续寻求替代治疗方法，并继续鼓励使用最新的结核病药物进行治疗。黑孜然(Nigella sativa)种子含有含有活性化合物的精油，如百里香对苯二酚、油树脂、类黄酮、生物碱、皂苷、单宁和萜类，这些化合物可以作为抗菌药物。本研究旨在确定芥蓝种子提取物对结核分枝杆菌H37RV和耐多药结核(MDR-TB)生长的抑制敏感性。本研究采用显微观察和药敏试验(MODS)方法。以70%甲醇为溶剂，采用浸渍法提取芥蓝。结果表明，结核分枝杆菌H37RV和耐多药结核分枝杆菌对浓度为5%和10%的苜蓿野蓟提取物敏感，对浓度为1%和3%的苜蓿野蓟提取物耐药。摘要:结核(TB) adalah penyakit menular yang disebabkan oleh巴克特里结核分枝杆菌。Penyakit ini menimbulkan dampak kematian yang cuup mengkhawatirkan。Penyakit tersebut dapat dicegah dan diobati。黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳，黑木耳。结核分枝杆菌H37RV与耐多药结核(MDR-TB)。孟古那坎方法:显微观察及药敏试验。黄芪、黄芪、黄芪、黄芪、黄芪、黄芪、黄芪、黄芪、黄芪。哈氏结核分枝杆菌H37RV株为TB-MDR，克氏结核分枝杆菌H37RV株为敏感株，耐药株占10%，耐药株占3%。
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