SINTESIS PERAK NANOPARTIKEL MELALUI REDUKSI SPONTAN MENGGUNAKAN REDUKTOR ALAMI EKTRAK KULIT LEMON SERTA KARAKTERISASINYA SEBAGAI ANTIFUNGI DAN ANTIBAKTERI

Abstract

Silver nanoparticle, Ag 0 , is a broad spectrum antimicrobial agent with a very low risk of resistance. In this study, silver nanoparticles, Ag 0 , were synthesized through the spontaneous reduction of Ag + solution using a natural reducing agent of lemon peel extract. Extraction was carried out at 60 o C for 10 minutes in a water solvent. The extraction of lemon peel involves varying the mass of the lemon peel powder, namely 2.5; 5; 7.5; and 10 grams to produce varying concentrations of reducing agents. Synthesis of silver nanoparticles was carried out at 60 o C for 20 minutes by mixing 0.1 M Ag + solution with the extract solution with various concentrations. The result of the synthesis is a dark brown to blackish colloidal solution which indicates the formation of Ag 0 from micro to nanoparticles in the solution. The concentration of lemon peel extract is directly proportional to the amount of Ag 0 formed, which is characterized by the colour of the solution. The Ag 0 solution showed its effectiveness as an antifungal under five days of observation. The Ag 0 solution also exhibits highly effective antibacterial properties based on testing against E. coli. ‘