{"title":"The Properties of Immobilized Invertase Onto a New Support Material; Poly(Methacrylamide/Maleic Acid) Copolymeric Hydrogel","authors":"H. Öztop, Fatma Banu Çatmaz, Dursun Saraydın","doi":"10.2478/achi-2018-0019","DOIUrl":null,"url":null,"abstract":"Abstract Poly (methacrylamide / maleic acid) PM/MA and poly (methacrylamide) PM hydrogels were prepared aiming to be used as a support for invertase. Spectrophotometric, thermal analysis methods, swelling and diffusion experiments were used for the characterization of hydrogels. The swelling of PM/MA was higher than that of PM in water. The diffusion of water within the hydrogel was found to be non-Fickian. Invertase was immobilized onto PM and PM/MA (samples named PM-I and PM/MA-I respectively). The optimum pH values were found to be; 6.0, 5.0 and 5.5 for free invertase, PM-I and PM/MA-I respectively. The optimum temperature values were found to be 30 °C, 35 °C and 40 °C for free invertase, PM-I and PM/MA-I respectively. The Michaelis constant (Km) and maximum velocity of the enzymes (Vmax) were Km: 11,75 mM, Vmax: 1,95 μmol min−1 for free invertase, Km: 67,24 mM, Vmax: 60,6 μmol min−1 for PM-I and Km: 74,55 mM, Vmax: 18,12 μmol min−1 for PM/MA-I. PM/MA-I showed excellent thermal, operational and storage stability.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"139 1","pages":"307 - 328"},"PeriodicalIF":0.4000,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Chemica Iasi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/achi-2018-0019","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 2
Abstract
Abstract Poly (methacrylamide / maleic acid) PM/MA and poly (methacrylamide) PM hydrogels were prepared aiming to be used as a support for invertase. Spectrophotometric, thermal analysis methods, swelling and diffusion experiments were used for the characterization of hydrogels. The swelling of PM/MA was higher than that of PM in water. The diffusion of water within the hydrogel was found to be non-Fickian. Invertase was immobilized onto PM and PM/MA (samples named PM-I and PM/MA-I respectively). The optimum pH values were found to be; 6.0, 5.0 and 5.5 for free invertase, PM-I and PM/MA-I respectively. The optimum temperature values were found to be 30 °C, 35 °C and 40 °C for free invertase, PM-I and PM/MA-I respectively. The Michaelis constant (Km) and maximum velocity of the enzymes (Vmax) were Km: 11,75 mM, Vmax: 1,95 μmol min−1 for free invertase, Km: 67,24 mM, Vmax: 60,6 μmol min−1 for PM-I and Km: 74,55 mM, Vmax: 18,12 μmol min−1 for PM/MA-I. PM/MA-I showed excellent thermal, operational and storage stability.
摘要制备了聚甲基丙烯酰胺/马来酸PM/MA和聚甲基丙烯酰胺PM水凝胶作为转化酶的载体。采用分光光度法、热分析法、溶胀和扩散实验对水凝胶进行表征。PM/MA的溶胀率高于PM在水中的溶胀率。水凝胶中水的扩散是非菲克式的。将Invertase固定在PM和PM/MA(样品分别命名为PM- i和PM/MA- i)上。发现最适pH值为;游离转化酶、PM- i和PM/MA-I分别为6.0、5.0和5.5。发现游离转化酶PM- i和PM/MA-I的最适温度分别为30℃、35℃和40℃。酶的米切里斯常数(Km)和最大速度(Vmax)分别为:游离转化酶Km: 11、75 mM, Vmax: 1.95 μmol min - 1; PM- i酶Km: 67、24 mM, Vmax: 60、6 μmol min - 1; PM/MA-I酶Km: 74、55 mM, Vmax: 18、12 μmol min - 1。PM/ ma - 1表现出优异的热稳定性、操作稳定性和存储稳定性。
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