P. Neubauer, C. Wolff, M. Hecker, K. Hofmann, L. Meyer, P. Kruschke, H. Heinrich
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引用次数: 17
Abstract
The use of the expensive IPTG for induction of the tac-promoter is not practicable at the industrial scale because of the large amounts necessary for induction. We developed a system in which IPTG as inducer was replaced by lactose which induces the tac-promoter with the same efficiency. In E. coli CW3011 (pOF1.0) lactose is utilized as inducer and carbon source even in the presence of glucose, presumably as a consequence of the overproduction of lac-permease.
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