Powder diffraction studies on proteins: An overview of data collection approaches

I. Margiolaki, J. Wright, A. Fitch, G. Fox, A. Labrador, R. Dreele, K. Miura, F. Gozzo, M. Schiltz, C. Besnard, F. Camus, P. Pattison, D. Beckers, T. Degen
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引用次数: 17

Abstract

Abstract. Following the seminal work of Von Dreele, high quality powder X-ray diffraction studies on proteins are being established as a valuable complementary technique to single-crystal measurements. Several studies using a variety of experiments approaches have been reported in the literature, including high-resolution studies employing parallel beam geome-try and high intensity measurements using position sensitive detectors. The choice of the optimum instrumental configuration depends on a number of competing factors such as the amount of sample available, its radiation sensitivity, and the quality of the data required for data analysis, e.g. angular resolution, the extent of the data in d-spacing, or the number of patterns required to explore the protein’s behaviour at different temperatures, or under differ-ent crystallisation conditions, etc. Here we discuss several advantages and disadvantages of different data collection methods followed for selected examples of small proteins.
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蛋白质的粉末衍射研究:数据收集方法综述
摘要在Von Dreele的开创性工作之后,高质量的粉末x射线衍射研究正在被建立为单晶测量的有价值的补充技术。文献中已经报道了几种使用各种实验方法的研究,包括采用平行束几何试验的高分辨率研究和使用位置敏感探测器的高强度测量。最佳仪器配置的选择取决于许多竞争因素,例如可用样品的数量,其辐射灵敏度,以及数据分析所需的数据质量,例如角分辨率,d间距数据的范围,或探索蛋白质在不同温度或不同结晶条件下的行为所需的模式数量等。在这里,我们讨论了不同的数据收集方法的几个优点和缺点,然后选择小蛋白质的例子。
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来源期刊
CiteScore
1.47
自引率
0.00%
发文量
0
审稿时长
3 months
期刊介绍: Zeitschrift für Kristallographie International journal for structural, physical, and chemical aspects of crystalline materials ISSN 0044-2968 Founded in 1877 by Paul Groth Zeitschrift für Kristallographie is one of the world’s oldest scientific journals. In original papers, letters and review articles it presents results of theoretical or experimental study on crystallography.
期刊最新文献
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