Ultrastructural analysis of erythroid colonies in diffusion chambers in irradiated rats. Effect of cytosine arabinoside administration on donor rats.

Z. Ben-ishay
{"title":"Ultrastructural analysis of erythroid colonies in diffusion chambers in irradiated rats. Effect of cytosine arabinoside administration on donor rats.","authors":"Z. Ben-ishay","doi":"10.1111/J.1600-0609.1975.TB02709.X","DOIUrl":null,"url":null,"abstract":"Ultrastructural observations of erythroid colonies in diffusion chambers implanted in lethally irradiated rats are reported. The donor cells were obtained from rats treated with cytosine arabinoside (250 mg/kg body weight) 6 h prior to seeding. The marrow of donor rats contained a high proportion of stem cells committed to the erythroid line. 9 days after implantation, vast numbers of erythroid colonies developed in the diffusion chambers. The erythroid cells were observed arranged around a central macrophage resembling erythroid islands in bone marrow. Erythroid colonies most probably differentiated from committed stem cells in the inoculum. It is postulated that development of erythroid colonies in diffusion chambers is conditioned by, among other factors, the presence of a special type of macrophages. These macrophages have the same function as those of central reticular cells (CRC) in bone marrow erythroid islands.","PeriodicalId":21489,"journal":{"name":"Scandinavian journal of haematology","volume":"10 1","pages":"369-76"},"PeriodicalIF":0.0000,"publicationDate":"2009-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"12","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scandinavian journal of haematology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1600-0609.1975.TB02709.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 12

Abstract

Ultrastructural observations of erythroid colonies in diffusion chambers implanted in lethally irradiated rats are reported. The donor cells were obtained from rats treated with cytosine arabinoside (250 mg/kg body weight) 6 h prior to seeding. The marrow of donor rats contained a high proportion of stem cells committed to the erythroid line. 9 days after implantation, vast numbers of erythroid colonies developed in the diffusion chambers. The erythroid cells were observed arranged around a central macrophage resembling erythroid islands in bone marrow. Erythroid colonies most probably differentiated from committed stem cells in the inoculum. It is postulated that development of erythroid colonies in diffusion chambers is conditioned by, among other factors, the presence of a special type of macrophages. These macrophages have the same function as those of central reticular cells (CRC) in bone marrow erythroid islands.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
辐照大鼠扩散室红系菌落超微结构分析。阿糖胞嘧啶给药对供体大鼠的影响。
报道了致死性辐照大鼠扩散室中红系菌落的超微结构观察。供体细胞来自于播种前6小时用阿糖胞嘧啶(250 mg/kg体重)处理的大鼠。供体大鼠的骨髓中含有高比例的红系干细胞。植入后第9天,扩散室中出现大量红系菌落。红细胞围绕中央巨噬细胞排列,类似骨髓中的红细胞岛。红系菌落很可能是从接种物中的固定干细胞分化而来的。据推测,扩散室中红系菌落的发育是由一种特殊类型的巨噬细胞的存在等因素决定的。这些巨噬细胞与骨髓红系岛的中枢网状细胞(CRC)具有相同的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Pyridoxine-responsive primary acquired sideroblastic anaemia. In vitro and in vivo effects of vitamin B6 on decreased 5-aminolaevulinate synthase activity. Permanent fixation of lymphoid cells exposed to dye exclusion test. Acute Renal Failure in Hereditary Spherocytosis. Report of a Case Studies on lymphocytes XIII. Nuclear volume measurement as a rapid approach to estimate proliferative fraction. The Influence of Technical Factors on the NBT Test
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1