CARBAMATE AND ORGANOPHOSPHATE RESIDUE ANALYSIS IN VEGETABLES BY RAPID BIOASSAY PESTICIDE RESIDUE

Janaki Budha, Prativa Sapkota, A. Acharya, Rakshya Bhandari, Narendra B. Singh
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Abstract

Farmers are using pesticides without or with little knowledge about their doses, rate, waiting periods, residual health hazards, etc. It is necessary to have good knowledge of safe consumption for both producers and consumers. The purpose of this study was to analyze the residue percentage in vegetables that reach the central vegetable market, Kalimati fruits and vegetable market, Kathmandu, from where we collected a total of 80 samples with a purposive sampling method and tested in the laboratory, 40 samples were tested for organophosphate (OP), and 40 for carbamate (C) residue percentage, using the RBPR kit. The highest and lowest OP% were found in cauliflower of Dhading (77.09%C) and okra of Chitwan (0.11%C) respectively. The highest and lowest carbamate% were found in the bitter gourds of Dhading (22.82%) and okra of Chitwan (0.31%) respectively. Two samples of cauliflower (77.09% OP) and bitter gourd (50.13% OP) from Dhading were observed to have an inhibiting percentage of more than 45% which meant unsafe for consumption. This shows that among the 100% samples, 2.50% are unsafe for consumption and others are safe. For cent % safe consumption, the waiting period needs to be followed properly before harvesting and consumption.
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快速生物测定法分析蔬菜中氨基甲酸酯和有机磷残留
农民在使用农药时不了解或很少了解农药的剂量、用量、等待期、残留的健康危害等。生产者和消费者都有必要对安全消费有良好的了解。本研究的目的是分析到达加德满都Kalimati水果和蔬菜市场中心蔬菜市场的蔬菜中的残留百分比,我们采用目的抽样方法收集了80个样品并在实验室进行了测试,其中40个样品使用RBPR试剂盒进行了有机磷酸酯(OP)和氨基甲酸酯(C)残留百分比的测试。有机磷含量最高的是哈定菜花(77.09%),最低的是Chitwan秋葵(0.11%)。氨基甲酸酯含量最高的是哈丁苦瓜(22.82%),最低的是Chitwan秋葵(0.31%)。来自达定的菜花(77.09% OP)和苦瓜(50.13% OP) 2个样品的抑制率均大于45%,不安全食用。这表明在100%的样品中,有2.50%的样品不安全,其余的是安全的。为了保证百分百的安全食用,在收获和食用前,需要遵循适当的等待期。
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