Hexavalent chromium reduction and 16S rDNA identification of bacteria isolated from a Cr (VI) contaminated site

A. Das, Susmita Mishra
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引用次数: 7

Abstract

A Gram-positive, hexavalent chromium [chromate: Cr (VI)]-resistant & reducing bacterium, isolated from sukind chromite mines, jajpur, India, was identified as a Brevibacterium casei(Gene Bank Accession Number: EU781952) by gene sequence homology. The strain(designated as APD15) could tolerate chromium up to a maximum concentration of 500 ppm, at optimum temperature and pH 30C and 7 for maximum chromium reduction. Agar supplemented with 100g Cr (VI)/ml as K2Cr2O7 and 0.5% (w/v) dextrose used as a carbon source. The results of the study indicated removal of more than 94% chromium (VI) by Brevibacterium caseidetermined by diphenylcarbazide colorimetric assay.
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铬(VI)污染场所分离细菌的六价铬还原和16S rDNA鉴定
从印度jajpur的sukind铬铁矿中分离到一株革兰氏阳性的六价铬[chromate: Cr (VI)]耐药还原细菌,经基因序列同源性鉴定为干酪短杆菌(brebacterium casei)(基因库登录号:EU781952)。该菌株(命名为APD15)可以耐受最高浓度为500 ppm的铬,在最佳温度和pH为30℃和7的最大铬还原。琼脂中添加100g Cr (VI)/ml的K2Cr2O7和0.5% (w/v)的葡萄糖作为碳源。研究结果表明,用二苯脲比色法测定短杆菌对铬(VI)的去除率超过94%。
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