Remediation of Hepatic Fibrosis as a Result of the use of CCl4 and Ethanol by Chitosan Nano-Green Tea Extract: Quantification and Ultrastructural Studies

Abstract

Antioxidant effects of Chitosan nano-green tea on hepatic fibrosis was studies ultrastrucutrally with detailed quantification comparing the percentage differences between the damaged done by CCl4 and ethanol effects and the rehabilitation by the use of Chitosan nano-green tea’s antioxidant effect. Especially in demolishing the damaging effects of CCl4 and ethanol on both the cell cytoplasm and the ECM. Chitosan nano-green tea exhibited several beneficial activities. Previously, we briefly reported that Chitosan nano-green tea completely demolishes hepatofibrosis in experimental models, but it was a short account and on one selected area. However, in this report we try to give an extensive elaboration on such effect and some details regarding the various aspects of cytoplasm and organelles as well as the ECM part. The 200 to 250 nm sized chitosan encapsulated GTE particles were used targeting rat liver fibrosis after being treated with CCl4 and ethanol doses for three weeks. Our data indicates that chitosan nano-GTE-induced a great change in demolishing the ECM protein fibrous materials and had left the area extremely smooth and clean. Damaged cell organelles were back to normal appearance and functions, cell cytoplasm damaged parts were highly healed up, parenchyma ECM were close to normalization with gentle removal of protein fibers which led to the smoothness of the field. This identification may explain the multiple therapeutic and anti-fibrotic activities of the nano-GTE.