Th-1 Directing Immunostimulatory Effect of Calcined Jade Having Naturally Formed Nanoparticles in Swiss Albino Mice

Abstract

In present study, we evaluated the modulating potential of Calcined Jade (CJ) on cell mediated immunity in Swiss albino mice. CJ is a metasilicate of Calcium and magnesium prescribed along with other herbal formulations in folk medicine for various purposes. CJ was administered orally to the animals at a dose of 50, 75, 100 and 200 µg/kg body wt. for 10 days; modulation of the acquired immune responses was studiedin mice. In CJ treated animals, cell mediated immune response was observed by estimation of significant increase (p<0.01) in Delayed type hypersensitivity (DTH) response, Th-1 cytokines namely IL-2/IFN-γ and TNF-α in spleenocytes. We also evaluated the effect of CJ on macrophage surface antigen markers (CD-80/86) and found increased expression of CD-80. We report stimulating effect of CJ on Th-1 type of immune responses which may partly be due to the formation of nano particles during calcination process and further experiments using isolated nano particles may further validate the role of nano particles.