Quantitative Analysis of AntiHevea brasiliensis Antibody Cross-Reactivity against Taraxacum kok-saghyz Latex Proteins Demonstrates Significantly Reduced Antibody Recognition

Julian T. Dafoe, Fang Huang, T. C. Yang
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Abstract

The annual plant Taraxacum kok-saghyz, commonly called Russian dandelion, has recently gained commercial attention as an alternative source to the rubber tree, Hevea brasiliensis, for natural rubber latex. While producing rubber of equivalent quality, the potential application of TKS latex for hypoallergenic products remains to be determined. In order to quantify the allergenicity of TKS latex proteins, we compared the extent of anti-Hevea latex polyclonal and monoclonal antibody cross-reactivity toward Hevea latex proteins to that of TKS latex proteins by quantitative ELISA methods and semi-quantitative image densitometry. ELISA measurement of polyclonal antibody recognition toward TKS latex proteins was on the order of one-tenth relative to equal amounts of Hevea latex protein, while recognition by the monoclonal antibody was below 2%. Immunoblots confirmed significantly reduced polyclonal antibody recognition toward TKS latex proteins, in the same range as the cross-reactivity exhibited toward the rubber-producing plant, Lactuca sativa and Glycine max, a non-rubber-producing plant. Despite the presence of cross-reactivity, these quantitative results support TKS as an alternative source of latex that may require lessintensive processing for the reduction of antigenic proteins in the manufacture of hypoallergenic natural rubber latex products.
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抗巴西橡胶树抗体与Taraxacum kok-saghyz乳胶蛋白交叉反应性的定量分析表明抗体识别显著降低
一年生植物Taraxacum koko -saghyz,通常被称为俄罗斯蒲公英,最近作为橡胶树巴西橡胶树(Hevea brasiliensis)的天然胶乳的替代来源而引起了商业关注。在生产同等质量的橡胶的同时,TKS乳胶在低过敏性产品中的潜在应用仍有待确定。为了量化TKS胶乳蛋白的致敏性,我们采用定量ELISA法和半定量图像密度法比较了抗Hevea胶乳蛋白的多克隆抗体和单克隆抗体与TKS胶乳蛋白的交叉反应程度。ELISA测定的多克隆抗体对TKS乳胶蛋白的识别率为等量Hevea乳胶蛋白的十分之一左右,而单克隆抗体的识别率低于2%。免疫印迹证实,多克隆抗体对TKS乳胶蛋白的识别显著降低,其交叉反应性与产胶植物乳胶树(Lactuca sativa)和非产胶植物甘氨酸(Glycine max)的交叉反应性相同。尽管存在交叉反应性,但这些定量结果支持TKS作为乳胶的替代来源,在生产低过敏性天然橡胶乳胶产品时,可能需要较少的密集加工来减少抗原蛋白。
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