Isolation and screening of different isolates of Aspergillus for amylases production

V. Morya, D. Yadav
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引用次数: 6

Abstract

The diversity of Aspergillus from soil of Kusmi Forest, an Indian subtropical Teak forest at Gorakhpur resulted in characterizing a total of 415 isolates which were then subjected to amylases production. The preliminary amylases production revealed a total of 14 promising isolates of Aspergillus comprising of ten different strains of A. flavus, two strains of A. tamarii, one strain each of A. niger and A. awamori which are characterized further. The optimum temperature of amylases produced from 14 isolates ranged from 30 to 60 C while thermal stability observed was in the range of 50 to 80 C for 30 minutes. The pH optima ranged from 5.0 to 8.6 and the Km values calculated ranged from 0.60 to 2.85 mg/ml.
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不同产淀粉酶曲霉的分离与筛选
对印度戈拉克布尔的亚热带柚木林Kusmi森林土壤中曲霉的多样性进行了分析,鉴定出415株曲霉,然后对其进行淀粉酶生产。初步淀粉酶生产结果显示,共有14株有潜力的曲霉分离株,包括10株黄曲霉、2株塔马瑞曲霉、1株黑曲霉和1株川曲霉。14株淀粉酶的最适温度为30 ~ 60℃,热稳定性为50 ~ 80℃,热稳定性为30 min。pH的最优值为5.0 ~ 8.6,Km的计算值为0.60 ~ 2.85 mg/ml。
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