PENENTUAN KADAR POLIFENOL EKSTRAK TEH KEMASAN DENGAN METODE REMASERASI MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS

Hadi Barru Hakam Fajar Siddiq, Jati Riyuwani, Rizka Della Yunita Dewi
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Abstract

The purpose of this research is determine the polyphenol content of various brand of packaged tea. Sample were used five sample. Polyphenol compounds of packaged tea were extracted using remaseration method with ethanol 70%. Qualitative test were used using FeCl3 reagent and quantitative test polyphenol content were determined using UV-Vis spectrophotometry method with Folin Ciocalteau reagent. Galic acid was used as comparator in this research. The result of qualitative test is all sample of brewed tea containing polyphenol. Polyphenol content sample A, B, C, D, and E with remaseration method were respectively 0,541; 0,557; 0,474; 0,557; and 0,428%(b/b).
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用UV-VIS光谱仪测定包装茶叶提取物的多酚含量
本研究的目的是测定不同品牌包装茶中多酚的含量。样本分别采用5个样本。采用70%乙醇再发酵法提取包装茶中的多酚类化合物。定性试验采用FeCl3试剂,定量试验采用福林试剂紫外-可见分光光度法测定多酚含量。本研究以没食子酸为比较剂。定性检测结果为泡茶样品均含有多酚。复温法样品A、B、C、D、E的多酚含量分别为0.541;0557;0474;0557;和0428% (b / b)。
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