Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2

Abstract

Background: The increase emergence and dissemination of antibiotic resistant Salmonella strains may contribute to a great risk on public health and impair classical treatment regime. The present study is aimed to investigate the prevalence of NTS in feces of broilers in four provinces in Iraq, and the resistance profile of isolates to clinically important antibiotics. Method: a total of 185 fecal samples were collected during the period from October2017 to March2018, from 28 broiler farms distributed in four governorates (Al- Najaf Al-Ashraf, AL-Qadyssia, Al-Muthana, and Babylon). Suspected Isolates of Salmonella were assayed for, detection of invA gene PCR, antibiotic susceptibility by Vitek-2. Results: The prevalence of NTS infection in 28 examined poultry flocks was 75,7%. High prevalence rate of NTS was observed in Babylon (91,7%), followed by Al-Qadyssia (72,7%) and Al-Muthana (70%), which significantly differed (P ≤ 0.01) than reported in AL- Najaf (50%). While the isolation rate was 21.6%. All Salmonella isolates were positive for the invA gene. The majority of isolates were resistant to Minocycline (87.5%) and Ciprofloxacin (85%) with P value ≤ 0.01. in addition to these, 55% of strains were resistant to Pipracillin, 52,5% to Ticarcillin (p ≤ 0.05), and 37,5% to Gentamycin. Fortunately, all isolates were susceptible to Amikacin, Meropenem, Imipenem, Piperacillin/Tazobactam and Ticarcillin/Clavulanic acid, followed by 97.5% of strains were sensitive to Ceftazidime, Cefepime and Aztreanam. Twenty (50%) isolates were exhibited multiple resistance to three or more different antibacterial classes with different resistance profiles. In conclusion, high prevalence of Salmonella and MDR Salmonella was found in broiler farms located in four Iraqi provinces.