{"title":"RP HPLC determination of benzhexol hydrochloride in tablet formulations and urine","authors":"R. Farag, Ashraf M. Ahmed","doi":"10.21608/ABSB.2010.7380","DOIUrl":null,"url":null,"abstract":"Anew, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of benzhexol hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 %v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mmi´4 mmi.d.). The flowrate was 0.6 ml/min and UV detection at 254nm. The retention time for benzhexol hydrochloride was 7.4 min. The calibration curve was linear up to 40iÂg/mL. The mean recovery for benzhexol hydrochloride is 101.16. The assaywas precisewithin day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.","PeriodicalId":7823,"journal":{"name":"Analytical chemistry, an Indian journal","volume":"57 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical chemistry, an Indian journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/ABSB.2010.7380","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Anew, rapid and sensitive reverse phase HPLC method was developed and validated for the determination of benzhexol hydrochloride in tablet formulations and urine. The mobile phase used acetonitrile and water, (50 %v/v) adjust pH to 5 using phosphoric acid. The separation was achieved on C18 reversed-phase column (250 mmi´4 mmi.d.). The flowrate was 0.6 ml/min and UV detection at 254nm. The retention time for benzhexol hydrochloride was 7.4 min. The calibration curve was linear up to 40iÂg/mL. The mean recovery for benzhexol hydrochloride is 101.16. The assaywas precisewithin day and between days. The method provided excellent sensitivity, recovery, accuracy and reproducibility in therapeutic or toxic concentrations. Common excipients do not interfere.
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