Diversity of Psychrophilic Colonies and Their Biotechnological Potential

M. Sidorenko, Daria A. Rusakova
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The natural ecological sites of psychrophiles are numerous and varied. Psychrophiles form a permanent microflora of eternal cold regions, polar regions and oceans. Bacteria belonging to this group are found in soil, water or associated with plants and animals. An important site for psychrophilic microorganisms is a low-temperature water reservoir. At present, many new genera of psychrophiles and psychrotrophs have been derived from the bottom sediments and sea waters of the Arctic and Antarctic and described. Psychrophilic microorganisms are found in caves and in ancient ice crystal structures. The latter testifies to the very possibility of the super-long anabiosis phenomenon, as well as vital capacity preservation without division for a long period of time. Psychrophiles do not have a single form, they belong to at least several phylogenetic groups. Psychrophilic forms are found among the representatives of a large number of genera. There are no common physiological and biochemical parameters typical of psychrophilic bacteria. They comprise rods, cocci, vibrios, gram-negative and gram-positive bacteria, bacteria that produce and do not produce spores, strict aerobes, facultative and strict anaerobes. We lay greater emphasis on the diversity of psychrophilic bacteria capable of producing industrially important enzymes. The review considers bacteria belonging to the genera Vibrio and Aliivibrio, Pseudomonas, Achromobacter, Arthrobacter, Pseudoalteromonas, Bacillus, Clostridium, Micrococcus, Psychrobacter, Psychromonas, Flavobacterium, and psychrophilic methanotrophic microorganisms. These bacteria enzymes are used in agriculture, biotechnology, pharmaceuticals and household chemicals, as well as other sectors of the national economy. Psychrophilic bacteria produce a chemical compound that can be used in medicine. For example, Pseudomonas antarctica contains a cluster of genes encoding microcin B, R-type pyocins, adenosylcobalamin, and pyrroloquinoline quinone. Thus, P. antarctica has antibiotic activity. Psychrobacter proteolyticus also has an antineoplastic action and secrets an extracellular cold-adapted metalloproteinase being able to inhibit the space-occupying process. Cold-active metalloproteinases are also widely used as detergents, in currying, food sector and molecular biology. The immunogenic Pal conformable protein was derived from the psychrophilic strain of The representatives of the genus Arthrobacter capable of metabolizing diuron and petroleum products have an important property. A. agilis produces a red pigment, a bacterioruberin-type carotenoid being interesting as an antioxidant. A. psychrochitiniphilus is promising for cleaning water areas, oil-polluted coastlines, as it decomposes oil and petroleum products. Flavobacterium limicola is a potential source of cold-active protease. This bacterium is characterized by an increase in protease secretion as temperature decreases. Thus, F. limicola can be used in environmental biotransformations and bioremediations. The psychrophilic bacteria of the genus Bacillus are the participants of active studies. Their cold-active enzymes have a high potential in various areas of biomedicine, immunology, decontamination, and various industrial applications. The antifreeze proteins of psychrophilic Clostridia are considered a promising biotechnological product for use in medicine, food, beauty products, fuel, and other industries. This study reviews literary sources and indicates that at present obligate and facultative psychrophiles (psychrotrophs) and their cold-active enzymes are of scientific interest throughout the world. A significant part of the research is focused on a general understanding of the distribution of psychrophilic bacteria and a local study of enzymatic activity. A further study of psychrophilic microorganisms producing enzymes at low temperatures will reveal new ways for the development of biotechnologies in various sectors of the national economy. The paper contains 94 References. The Authors declare no conflict of interest.","PeriodicalId":37153,"journal":{"name":"Vestnik Tomskogo Gosudarstvennogo Universiteta-Biologiya","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Vestnik Tomskogo Gosudarstvennogo Universiteta-Biologiya","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17223/19988591/58/2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

Psychrophilic bacteria are a large group of microorganisms that prevail in low-temperature ecosystems. Psychrophilic bacteria have undergone a number of adaptations that help them exist in such conditions. One of such adaptations is the use of enzymes with a high specific activity at low temperatures. Such enzymes are usually called “cold-active.” These enzymes have potential applications in biotechnology and industry. In our review, we considered individual genera of psychrophilic bacteria, current global trends in the study of cold-active enzymes, their applications, and place in industrial biotechnology. Thus, the main goal of this study was to explore the diversity of psychrophilic bacteria, as well as opportunities of their application in biotechnology. The natural ecological sites of psychrophiles are numerous and varied. Psychrophiles form a permanent microflora of eternal cold regions, polar regions and oceans. Bacteria belonging to this group are found in soil, water or associated with plants and animals. An important site for psychrophilic microorganisms is a low-temperature water reservoir. At present, many new genera of psychrophiles and psychrotrophs have been derived from the bottom sediments and sea waters of the Arctic and Antarctic and described. Psychrophilic microorganisms are found in caves and in ancient ice crystal structures. The latter testifies to the very possibility of the super-long anabiosis phenomenon, as well as vital capacity preservation without division for a long period of time. Psychrophiles do not have a single form, they belong to at least several phylogenetic groups. Psychrophilic forms are found among the representatives of a large number of genera. There are no common physiological and biochemical parameters typical of psychrophilic bacteria. They comprise rods, cocci, vibrios, gram-negative and gram-positive bacteria, bacteria that produce and do not produce spores, strict aerobes, facultative and strict anaerobes. We lay greater emphasis on the diversity of psychrophilic bacteria capable of producing industrially important enzymes. The review considers bacteria belonging to the genera Vibrio and Aliivibrio, Pseudomonas, Achromobacter, Arthrobacter, Pseudoalteromonas, Bacillus, Clostridium, Micrococcus, Psychrobacter, Psychromonas, Flavobacterium, and psychrophilic methanotrophic microorganisms. These bacteria enzymes are used in agriculture, biotechnology, pharmaceuticals and household chemicals, as well as other sectors of the national economy. Psychrophilic bacteria produce a chemical compound that can be used in medicine. For example, Pseudomonas antarctica contains a cluster of genes encoding microcin B, R-type pyocins, adenosylcobalamin, and pyrroloquinoline quinone. Thus, P. antarctica has antibiotic activity. Psychrobacter proteolyticus also has an antineoplastic action and secrets an extracellular cold-adapted metalloproteinase being able to inhibit the space-occupying process. Cold-active metalloproteinases are also widely used as detergents, in currying, food sector and molecular biology. The immunogenic Pal conformable protein was derived from the psychrophilic strain of The representatives of the genus Arthrobacter capable of metabolizing diuron and petroleum products have an important property. A. agilis produces a red pigment, a bacterioruberin-type carotenoid being interesting as an antioxidant. A. psychrochitiniphilus is promising for cleaning water areas, oil-polluted coastlines, as it decomposes oil and petroleum products. Flavobacterium limicola is a potential source of cold-active protease. This bacterium is characterized by an increase in protease secretion as temperature decreases. Thus, F. limicola can be used in environmental biotransformations and bioremediations. The psychrophilic bacteria of the genus Bacillus are the participants of active studies. Their cold-active enzymes have a high potential in various areas of biomedicine, immunology, decontamination, and various industrial applications. The antifreeze proteins of psychrophilic Clostridia are considered a promising biotechnological product for use in medicine, food, beauty products, fuel, and other industries. This study reviews literary sources and indicates that at present obligate and facultative psychrophiles (psychrotrophs) and their cold-active enzymes are of scientific interest throughout the world. A significant part of the research is focused on a general understanding of the distribution of psychrophilic bacteria and a local study of enzymatic activity. A further study of psychrophilic microorganisms producing enzymes at low temperatures will reveal new ways for the development of biotechnologies in various sectors of the national economy. The paper contains 94 References. The Authors declare no conflict of interest.
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嗜冷菌落的多样性及其生物技术潜力
嗜冷细菌是在低温生态系统中普遍存在的一大群微生物。嗜冷细菌经历了许多适应,帮助它们在这样的条件下生存。其中一种适应是使用在低温下具有高比活性的酶。这种酶通常被称为“冷活性酶”。这些酶在生物技术和工业上具有潜在的应用前景。在我们的综述中,我们考虑了嗜冷细菌的个别属,目前全球冷活性酶的研究趋势,它们的应用,以及在工业生物技术中的地位。因此,本研究的主要目的是探索嗜冷细菌的多样性及其在生物技术中的应用机会。嗜冷动物的自然生态场所是众多而多样的。嗜冷菌在寒冷地区、极地地区和海洋中形成了永久的菌群。这类细菌存在于土壤、水中或与动植物有关的细菌中。低温水库是嗜冷微生物生存的重要场所。目前,从北极和南极的海底沉积物和海水中发现并描述了许多新属的嗜冷和嗜冷生物。在洞穴和古老的冰晶结构中发现了嗜冷微生物。后者证明了超长复苏现象的可能性,以及长时间不分裂的生命容量保存的可能性。嗜冷动物没有单一的形态,它们至少属于几个系统发育类群。在许多属的代表中发现了亲湿形式。嗜冷细菌没有共同的生理生化参数。它们包括杆状菌、球菌、弧菌、革兰氏阴性菌和革兰氏阳性菌、产生和不产生孢子的细菌、严格需氧菌、兼性和严格厌氧菌。我们更加重视能够生产工业上重要酶的嗜冷细菌的多样性。该综述考虑了属于弧菌属和alivibrio属、假单胞菌属、无色杆菌属、节杆菌属、假互变单胞菌属、芽孢杆菌属、梭状芽胞菌属、微球菌属、冷杆菌属、冷单胞菌属、黄杆菌属和嗜冷甲烷营养微生物的细菌。这些细菌酶用于农业、生物技术、制药和家用化学品以及国民经济的其他部门。嗜冷细菌产生一种可用于医药的化合物。例如,南极假单胞菌含有一簇编码微霉素B、r型脓毒蛋白、腺苷钴胺素和吡咯喹啉醌的基因。因此,南极菌具有抗生素活性。嗜冷杆菌也具有抗肿瘤作用,并分泌一种能够抑制占位过程的细胞外冷适应金属蛋白酶。冷活性金属蛋白酶还广泛应用于洗涤剂、咖喱、食品和分子生物学等领域。免疫原性Pal适形蛋白来源于嗜冷菌属的代表菌,具有代谢迪乌隆和石油产品的重要特性。a . agilis产生一种红色色素,一种细菌红蛋白类型的类胡萝卜素是一种有趣的抗氧化剂。嗜寒几丁杆菌可以分解石油和石油产品,有望用于清洁水域和受石油污染的海岸线。极限黄杆菌是冷活性蛋白酶的潜在来源。这种细菌的特点是温度降低时蛋白酶分泌增加。因此,该菌可用于环境生物转化和生物修复。芽孢杆菌属的嗜冷细菌是积极研究的参与者。它们的冷活性酶在生物医学、免疫学、去污和各种工业应用领域具有很高的潜力。嗜冷梭菌的抗冻蛋白被认为是一种很有前途的生物技术产品,可用于医药、食品、美容产品、燃料和其他行业。本研究回顾了文献资料,指出目前专性和兼性嗜冷菌及其冷活性酶在世界范围内具有重要的科学意义。研究的一个重要部分集中在对嗜冷细菌分布的一般理解和对酶活性的局部研究上。对低温产酶的亲冷微生物的进一步研究,将为国民经济各部门生物技术的发展开辟新的途径。本文包含94篇参考文献。作者声明无利益冲突。
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