Determination of Pregabalin in Tissues and Fluids by Using GC

Abstract

Pregabalin is an antiepileptic and analgesic drug, commercialized under the name of Lyrica and other names, generally used to treat neuropathic pain. The determination of pregabalin was quantified in tissues and fluids samples by using Gas chromatography coupled with mass spectrometry, isolation and precipitation protein by Ammonium Sulphate method. The limit of detection (LOD) is 200 ng and the limit of quantification (LOQ) in is 400 ng. Ibuprofen was used as internal standard, this methods has been applied in two cases of pregabalin.