Molecular Detection Of Virulence Gene From Nosocomial Staphylococcus Infection In Hospital Kuala Lumpur, Malaysian-A Brief Report

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) remains as the most nosocomial pathogen and is now the emerging problem of community-associated infections. In the present study 100 nasal samples from pediatric patients were subjected to bacteriological and molecular tests for the identification drug resistant Staphylococcus organisms and detection of responsible genes. It revealed that out of 100 nares specimens examined 25% were Staphylococcus aureus, 65% were coagulase-negative Staphylococcus (CONS), and 10% were positive other than Staphylococcus. Among 25 Staphylococcus aureus isolates, only 1 identified as MRSA. The MRSA carrier strain was characterized through sequencing and it was observed that the strains possesses SCCmec type III, Spa type t037 and sequence type 239 with presence of sea and fnbA genes. In conclusion MRSA carrier strain can represent the clonal distribution of locally prevalent MRSA clone. Therefore, a study with large number of strains from many regions is needed to confirm the predominant clones circulating in Malaysia.