Effects of Resveratrol on Oxidative Stress Injury Induced by Rapid-Pacingin Isolated Rabbit Hearts

Xiaoqin Hu, Wang Zhirong, Z. Chaoqun, Z. Zhuoqi, S. Hong, Chen Mingyue
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引用次数: 1

Abstract

Oxidative stress injury plays an important role in the process of atrial remodeling. The mechanisms of oxidative stress injury in atrial by rapid pacing and the protective effects of resveratrol will be explored in this study. Thirty-two isolated rabbit hearts were produced by rapid atrial pacing. They were randomly divided into 4 groups: control group (Ctrl group), Rapid Atrial Pacing group (RAP group), Apocynin Pretreatment group (APO group) and Resveratrol Pretreatment group (RES group); each with 8 rabbits. At the end, the indexs of the oxdise stress were measured take advantage of the techniques of the immunohistochemistry, western blotting and reverse transcription PCR.
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白藜芦醇对快速起搏诱导的离体兔心脏氧化应激损伤的影响
氧化应激损伤在心房重构过程中起重要作用。本研究将探讨快速起搏引起心房氧化应激损伤的机制及白藜芦醇的保护作用。采用快速心房起搏法制备32颗离体兔心脏。随机分为4组:对照组(Ctrl组)、快速心房起搏组(RAP组)、罗布麻素预处理组(APO组)、白藜芦醇预处理组(RES组);每个人有8只兔子。最后采用免疫组织化学、western blotting和反转录PCR技术检测氧化应激指标。
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