Yu Liu, N. Song, J. Dang, Hairong Chen, Dong Yang, Jian Gao, D. Song, Tianzhu Yang, H. Tian
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引用次数: 0
Abstract
This paper reports a hybrid electrokinetic-microfluidic chip, which combines electrophoresis (EP), dielectrophoresis (DEP) or AC electrothermal flow (ACEF), for isolating circulating tumor cells (CTCs) in physiological sample. While most electrokinetic techniques only function in low-conductivity buffers, hybrid electrokinetics enables effective operation in high-conductivity samples (1 S/m), which are compatible to physiological fluids such as blood, buffy coat and urine. Using a 3-electrode configuration and optimizing AC voltage, frequency and DC offset, we demonstrate that breast cancer cell can be localized within 10 sec in buffy coat without any pretreatment.
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