INVOLVEMENT OF MAIN OXIDATIVE STRESS MECHANISMS IN THE TOXICITY OF BENOMYL AND CARBENDAZIM IN RATS

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2013-01-01 DOI:10.16883/JFPIU.87601

Sadik Catalgol, B. Alpertunga

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引用次数: 5

Abstract

SUMMARY This study aimed to evaluate the role of oxidative stress in toxicity induced by BNL (benomyl) and its metabolite CBZ (carbendazim), which are systemic broad-spectrum benzimidazole fungicides. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rd), γ−glutamyl transpeptidase (GGT) and glutathione-S-transferase (GST) as well as the levels of malonyldialdehyde (MDA), being a lipid peroxidation marker and reduced glutathione (GSH) were measured in the liver, kidney, testis and brain tissues of the rats, after acute exposure of the two fungicides and their mixture. In all tissues, it was observed that MDA levels were increased and changes in antioxidant defense system were occurred. The induction of oxidative stress manifested as changes in the activity and/or levels of antioxidative parameters has been suggested as the mechanism by which BNL and CBZ induces their toxic effects. OZET Bu calismada, genis spektrumlu sistemik benzimidazol grubu fungusidlerden olan BNL (benomyl) ve metaboliti CBZ (carbendazim)’ nin toksik etki mekanizmalarinda oksidatif stresin rolu arastirildi. BNL, CBZ ve karisimlarina akut maruziyet sonrasi sicanlarin karaciger, bobrek, testis ve beyin dokularinda oksidatif stres gostergesi olarak lipid peroksidasyon son urunu malondialdehid (MDA) ve indirgenmis glutatyon (GSH) seviyeleri, superoksid dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHPx), glutatyon reduktaz (GSH-Rd), gama glutamil transpeptidaz (GGT) ve glutatyon-S-transferaz (GST) antioksidan enzim aktivitelerinin tayini yapildi. Tum dokularda MDA duzeylerinin arttigi ve antioksidan savunma sistemlerinde degisiklik oldugu gozlendi. BNL ve CBZ’nin toksisitesinde, antioksidan parametrelerin aktivite ve/veya duzeylerinde oksidatif stresin induksiyonu sonucu gorulen degisikliklerin rol oynadigi sonucuna varildi. Key words: Oxidative Stress, Benomyl, Carbendazim, Pesticide toxicity

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苯甲酰和多菌灵对大鼠毒性的主要氧化应激机制参与

摘要本研究旨在探讨氧化应激在苯并咪唑类广谱杀菌剂BNL (benomyl)及其代谢物CBZ (carbendazim)致毒中的作用。测定两种杀菌剂及其混合物急性暴露后大鼠肝脏、肾脏、睾丸和脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH- px)、谷胱甘肽还原酶(GSH- rd)、γ -谷氨酰转肽酶(GGT)和谷胱甘肽s -转移酶(GST)的活性以及脂质过氧化标志物丙二醛(MDA)和还原性谷胱甘肽(GSH)的含量。在所有组织中均观察到MDA水平升高，抗氧化防御系统发生改变。氧化应激的诱导表现为抗氧化参数的活性和/或水平的变化，这被认为是BNL和CBZ诱导其毒性作用的机制。[6] [1] [1] [1] [1] [1] [1] [1] [1] [1] [3] [1] [1] [3] [1] [3] [1] [4] [1] [4] [1] [4] [1] [4] [1] [4]BNL, CBZ, karisimlarina akut maruziyet sonrasi sicanlari karaciger, bobrek，睾丸，dokularinda oksidatif应激，gostergesi, olarak，脂质过氧化氢，ununu，丙二醛(MDA)，原谷胱甘肽(GSH)，超谷胱甘肽歧化酶(SOD)， katalaz (CAT)，谷胱甘肽过氧化氢(GSHPx)，谷胱甘肽还原(GSH- rd)， γ -谷氨酰胺转肽酶(GGT)，谷胱甘肽- s -转移(GST)抗谷胱甘肽酶活性，谷氨酸- s -转移(GST)。二甲基丙二胺二甲基丙二胺二甲基丙二胺二甲基丙二胺二甲基丙二胺二甲基丙二胺二甲基丙二胺二甲基丙二胺。研究结果表明:CBZ蛋白具有抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性、抗氧化活性和抗氧化活性。关键词:氧化应激，苯甲酰，多菌灵，农药毒性

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Journal of Faculty Pharmacy of Istanbul University

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