Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism

Journal of Faculty Pharmacy of Istanbul University Pub Date : 2016-11-22 DOI:10.16883/JFPIU.54980

Ş. H. Tekarslan, Ceren Alkim, C. Hunte, Z. P. Çakar

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Abstract

Abstract: The yeast Saccharomyces cerevisiae is a simple eukaryote and an excellent model organism for molecular biology. In this study, a NaCl-resistant S. cerevisiae mutant obtained by inverse metabolic engineering was used as a model to investigate responses and resistance behavior to NaCl, LiCl, KCl, TMA, spermine and sorbitol stresses., at physiological and genetic levels. The physiological spot test results revealed that the NaCl-resistant yeast mutant showed higher resistance to LiCl and NaCl. Gene expression analysis by qRT-PCR revealed that ENA6 and NHA1 genes of the mutant were induced in the absence and presence of LiCl and NaCl. The dysfunction of Na+/H+ antiporters are related to several diseases such as hypertension, epilepsy, postischemic myocardial arrhythmia, gastric and kidney disease, diarrhea, and glaucoma. Thus, the NaCl-resistant yeast mutant could be used to understand cellular sodium and lithium resistance mechanisms and the function of Na+/H+ antiporters also in higher eukaryotic organisms, including humans. Key words: Saccharomyces cerevisiae, stress resistance, salt-resistance , lithium resistance, NHA1

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以酿酒酵母为模式生物的细胞钠锂响应/抗性生理和遗传分析

摘要:酵母(Saccharomyces cerevisiae)是一种简单的真核生物，是分子生物学的优良模式生物。本研究以反代谢工程获得的耐NaCl酿酒酵母突变体为模型，研究了其对NaCl、LiCl、KCl、TMA、精胺和山梨醇胁迫的响应和抗性行为。在生理和基因水平上。生理斑点试验结果表明，酵母耐NaCl突变体对LiCl和NaCl具有较高的抗性。qRT-PCR基因表达分析显示，突变体的ENA6和NHA1基因在LiCl和NaCl存在和不存在的情况下均被诱导。Na+/H+反转运蛋白功能障碍与高血压、癫痫、缺血性心肌心律失常、胃和肾脏疾病、腹泻、青光眼等疾病有关。因此，耐盐酵母突变体可用于了解包括人类在内的高等真核生物的细胞耐钠和耐锂机制以及Na+/H+反转运蛋白的功能。关键词:酿酒酵母，抗逆性，耐盐性，耐锂性，NHA1

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Journal of Faculty Pharmacy of Istanbul University

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