PHYTOCHEMICAL STUDIES AND IN-VITRO ANTI-OXIDANT ACTIVITY OF BREYNIA RETUSA DENNST

Abstract

To investigate the phytochemical screening and antioxidant potential of ethanolic and chloroform extract of breynia retusa leaf & stem. The study was done by using various invitro methods such as 1, 1diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (N2O2) and Superoxide radical scavenging assays. Phytochemical screenings were performed by various standard procedures. Ethanolic and chloroform extract of 10-100μg/ml Breynia retusa leaf & stem showed high free radical scavenging activity as evidenced by the low IC50 values in DPPH (26μg/ml), in nitric oxide (31.2μg/ml) and in SOD (25.5μg/ml) radical scavenging assays at the concentration of 100μg/ml. The results of present comprehensive analysis demonstrated that ethanolic extract (100μg/ml) showed more significant anti-oxidant activity than chloroform extract of (100μg/ml) Breynia retusa leaf & stem. Breynia retusa could be used as a viable source of natural antioxidants and might be exploited for functional foods and neutraceutical applications.