PRODUCTION OF MICROBIOLOGICAL PROTEIN FEED FROM SWEET POTATO (Ipomoea batatas L. Lam) RESIDUE BY CO-CULTIVATION SACCHAROMYCES CEREVISIAE AND CANDIDA UTILIS

June 1 Pub Date : 2023-02-10 DOI:10.36899/japs.2023.3.0652
Y. L. Jin, F. Ding, W. L. Shen, Y. Fang, Z. L. Yi, L. Yang, H. Zhao
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Abstract

Sweet potato residue (SPR), a byproduct generated during starch extraction from sweet potato, is usually treated as an agro-waste, resulting in lower economic benefits and serious environmental pollution. To promote resource recycling, SPR was used as a starting material to prepare a microbiological protein feed (MPF) by co-cultivating Saccharomyces cerevisiae and Candida utilis . The cultivation conditions were investigated, and the optimum conditions were determined as follows: inoculation amount of S. cerevisiae , 5%; inoculation amount of C. utilis , 5%; water content of SPR, 75%; urea, 1%; cellulase, 0.5%; and isoamylase, 0.5%, at a culture temperature of 32°C. Under these conditions, true protein content in fermented SPR was enhanced to 18.08%, which was 6.34 times that of the original SPR. Amino acid composition and the essential amino acid index (EAAI) indicated the high nutritive value of MPF to cattle, rabbits, and lambs. This simple method provides a strategy for recycling and reusing agricultural residues in an economical and environment-friendly manner.
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酿酒酵母菌与实用假丝酵母共培养甘薯渣生产微生物蛋白饲料
甘薯渣(SPR)是甘薯淀粉提取过程中产生的副产品,通常作为农业废弃物处理,经济效益低,环境污染严重。为促进资源循环利用,以SPR为原料,通过酿酒酵母和实用念珠菌的共培养制备微生物蛋白饲料(MPF)。对培养条件进行了研究,确定了最佳培养条件:葡萄球菌接种量为5%;接种量:5%;SPR含水率75%;尿素,1%;纤维素酶,0.5%;异淀粉酶0.5%,培养温度32℃。在此条件下,发酵后的SPR真蛋白质含量达到18.08%,是原始SPR的6.34倍。氨基酸组成和必需氨基酸指数(EAAI)表明MPF对牛、兔和羔羊具有较高的营养价值。这种简单的方法提供了一种以经济和环境友好的方式回收和再利用农业残留物的策略。
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