Based on 16S rDNA Amplicon Sequencing and Flow Cytometry, the Relationship between Pueraria Improving Immunity and Intestinal Microorganism Was Analyzed

Abstract

The aim of this study was to explore the immune improvement mechanism of Pueraria based on 16S rDNA amplicon sequencing and flow cytometry, and to analyze the relationship between intestinal microbes and immunity, measuring the serum levels of TNF-α , IFN-γ and IL-6 w, determining the number of T and B lymphocytes in serum by flow cytometry. The changes of intestinal microflora in feces were sequenced by amplicon of 16S rDNA. The results showed that compared with the blank control group, all the doses of pueraria decreased inflammatory factors and improved the immune state of the body, and the low dose was the most significant (P < 0.05). Flow cytometry results showed that the number of T and B lymphocytes in serum of pueraria root extract in each dose group was significantly increased (P < 0.05), and the increase rate was most significant in low dose group (P < 0.01). 16S rDNA amplified sequencing results showed that pueraria root extract could specifically increase the abundance of immune-related intestinal microorganisms, such as Clostridium difficile, Roche, Parabacteroides dieldii , enterococcus, etc. The difference analysis of Alpha diversity index between groups suggested that pueraria root could improve the abundance and distribution uniformity of intestinal microorganisms. PCoA analysis indicated that pueraria could significantly change the species abundance and composition of intestinal microorganisms. Therefore, pueraria root can improve immune function, reduce inflammatory factors, increase the number of T and B lymphocytes, besides, it may play an immunomodulatory role by changing the intestinal microflora, such as parabens Diels, and increasing the diversity of intestinal microflora