Evaluation of Indian Peanut on the Induction of Caulogenic Buds i n Vitro

S. Raman, Dina Ermias Dagne, N. Malpathak, W. Gade
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Abstract

The present work has been undertaken to study the in vitro response in widely used cultivars WesternHB-55, TAG 24 and SB11 of peanut having desired agronomic characteristics. The mature embryo derived leaflet explants of all three peanut genotypes were found morphogenic in vitro. When comparing the effects of TDZ and BA with respect to cau- logenic response, it can be inferred that TDZ is a more potent inducer of caulogenesis in vitro than BA. All genotypes re- sponded equally to different concentrations of BA. WesternHB-55 responded better than others which results in high pro- liferation rate in vitro. The present study emphasizes on the usage of WesternHB-55 germplasm through adventitious or- ganogenesis pathway in transformation studies (biolistic gun approach), for the production of proteins, vaccines enzymes etc.
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印度花生离体诱导茎源芽的评价
研究了花生品种westnhb -55、TAG 24和SB11的离体反应。三种花生基因型的成熟胚衍生小叶外植体在离体培养中均有形态发生。当比较TDZ和BA对病因反应的影响时,可以推断TDZ是比BA更有效的体外致癌剂。所有基因型对不同浓度的BA反应相同。WesternHB-55反应较好,体外增殖率高。本研究重点介绍了western nhb -55种质资源在转化研究(生物枪方法)中通过不定或非器官发生途径的应用,用于生产蛋白质、疫苗、酶等。
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