Immunofluorescent and Immunogold Localization of Glutamate Dehydrogenase in the Nodules of Soybean

Abstract

Soybean nodules have been examined for localization of glutamate dehydrogenase, using a monospecific antiserum to the plant enzyme prepared from lupin nodules. By immunofluorescence techniques, glutamate dehydrogenase was located in the cytoplasm at the periphery of infected cells adjacent to air spaces. A more precise localization within soybean-nodule mitochondria was shown by immuno-electron microscopy using protein A-gold labelling of thin sections.