Pre-fusion events of sperm-oocyte interaction in the marine shrimp, Sicyonia ingentis

Wallis H. Clark Jr, Fred J. Griffin, Athula H. Wikramanayake
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引用次数: 10

Abstract

The non-motile sperm of Sicyonia ingentis, mixed with eggs by a spawning female, undergo a primary binding to the vitelline envelope (VE) of the oocyte. Once bound to the VE, sperm undergo exocytosis of the acrosomal vesicle, penetrate the VE, and secondarily bind to a surface coat that is closely associated with the oolemma. Unreacted sperm preincubated with solubilized VE components exhibit diminished binding to VEs in a concentration dependent manner. The ligand responsible for this binding is a carbohydrate moiety in the VE. The ligand preferentially binds to the anterior tip of unreacted sperm, as demonstrated with anti-VE polyclonal antibodies. Acrosome intact sperm will not bind to surface coats; however, acrosome reacted sperm do bind to surface coats via an externalized acrosomal granule.

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海虾精卵相互作用的预融合事件
由产卵的雌虫与卵混合而成的无运动精子与卵母细胞的卵黄膜(VE)进行初级结合。精子一旦与卵囊膜结合,就会经历顶体囊泡的胞外作用,穿透卵囊膜,然后与与卵囊膜密切相关的表面被膜结合。未反应的精子与溶解的VE成分预孵育表现出浓度依赖的方式减少与VE的结合。负责这种结合的配体是VE中的碳水化合物部分。如抗ve多克隆抗体所示,该配体优先结合未反应精子的前端。顶体完整精子不会与表面被毛结合;然而,顶体反应的精子确实通过外化顶体颗粒与表面被膜结合。
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