A Study Comparative of Some Haematological Parameters after Treatment by Extracted Octacosanol and Atorvastatin in Female Rats

Abstract

Hematological and serum parameters are a prerequisite to understand the normal functioning of the system and to confirm the nontoxic nature of the administered crude drug. Hematological parameters such as mean platelet volume (MPV) and RBCs count are associated with the increased risk for cardio vascular diseases CVD. Octacosanol is the major effective component of policosanol forming about (62.9%), it has antioxidative, lipid lowering, antithrombotic (antiplatelet) effects and provides protection against free radical associated diseases. Statins are the inhibitors of hydroxymethyl glutaryl coenzyme A (HMG CoA) reductase. They are mostly used to treat hyperlipidaemia. Statins such as atorvastatin and some of medical plants have shown their beneficial effect on the cardiovascular disease (CVD) by virtue of their lipid lowering, antioxidant and cardioprotective effects. The present study was designed to compare some of hematological parameters after treatment of extracted  octacosanol  and atorvastatin in female Norwegian rats. Thirty two females rat with (10-12) weeks old-and weighting (200-250 gm) were used in the current study. Policosanol was extracted from sugarcane that obtained from Mesan province. Octacosanol is determined in this extraction by gas chromatography – mass spectrometry (GC/MS). Animals were divided into 4 equal groups: G.1 (n=8) (Control) : only 0.5 ml/ animal of Dimethyl Sulphoxide (DMSO). G.2 (n=8) animals treated with (0.02 mg/ animal) of standard octacosanol . G.3 (n=8) animals treated with (0.02mg/ animal)  of octacocosanol extraction, G. 4 (n=8) treated with only  (0.02mg/ animal) of atorvastatine.  The treatment continued for 30 days. All animals were then sacrificed and blood samples were collected in EDTA tubes for measuring of hematological parameters. Results: all hematological parameters (RBCs, Hb, MCV, MCH and MCHC) in group treated with extracted octacosanol were nearby the control group except platelet count and MPV level were lower than those of control group, while platelet count and MPV were higher in extracted group than those in the Atorvastatin group (all P≤0.05). In conclusion: The antiplatelet activity of the extracted octacosanol is more than atorvastatin and without side effects. So the extracted octacosanol successfully used in prevention of platelet aggregation and thrombosis in patients with CVD.