A. Avram, C. Marculescu, C. Bălan, C. Voitincu, C. Pirvulescu, M. Volmer, Andrei C. Popescu, Mona Mihailescu, M. Avram
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引用次数: 3
Abstract
This paper presents the fabrication and characterization of an interdigitated microelectrode array (IDMEA) biosensor. Electrochemical impedance spectroscopy and cyclic voltammetry were used to study the cellular activities of B16 melanoma cell line C57BL, including the kinetics of cell adhesion and spreading on IDMEA. Impedance spectra of B16 melanoma cells on microelectrodes were obtained in cell 0.1 M PBS with 50 mM [Fe(CN)(6)](3-/4-) as redox species. Randles equivalent circuits were used to model the electrochemical processes. Impedance spectra allowed us to analyze the changes in the double layer capacitance and charge transfer resistance due to cell attachment on the interdigitated microelectrodes.
本文介绍了一种交叉指状微电极阵列(IDMEA)生物传感器的制备和表征。采用电化学阻抗谱和循环伏安法研究了B16黑色素瘤细胞系C57BL的细胞活性，包括细胞在IDMEA上的粘附和扩散动力学。在细胞0.1 M PBS中，以50 mM [Fe(CN)(6)](3-/4-)作为氧化还原物质，获得了B16黑色素瘤细胞在微电极上的阻抗谱。采用Randles等效电路模拟电化学过程。阻抗谱允许我们分析由于电池附着在交叉微电极上而引起的双层电容和电荷转移电阻的变化。
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